234 



Scientific Proceedings (115). 



equilibrium, though he also ascribes a specific stimulating effect 

 to the bicarbonate ion. To the present author, it seems that this 

 specific effect may be due entirely to the effect of the anion in 

 determining the permeability of the tissues to the kation. 



TABLE II. 











Sodium. 











V 



Per Kilo 













a 

 a 



H 



Body Weight 



Per 



100 cc. 





Ex. 



Salt. 



Concn. 









Remarks. 











Injec. 



Re- 



Plas- 













tained 



ma. 













Gr'ms 



Gr'ms 











N. 



min 











I 



NaHCOs 



0.9 



38 



O.694 



0.493 



442 



Twitching. 









44 



O.831 



O.631 



447 



Convulsions. 



5 



NaHCOs 



0.9 



40 



0.747 



0.730 



436 



Twitching. 



2 



NaHCOs 



0.9 



43 



O.783 



O.684 



514 



Twitching. Injected soln. contained 

 0.1 equivalent of K, Mg and Ca. 



3 



NaHCOs 



0.9 



40 



0.793 



O.709 



457 



Convulsions. Injected soln. con- 

 tained 1.0 equivalent of K, Mg 

 and Ca. 



4 



NaHCOj 



O.47 



68 



1. 140 



O.87 



420 



No twitching or tremor. Death due 

 to respiratory failure. 



5 



NaCl 



O.60 1 



35 



1 -45 



1.30 



493 



Convulsions. 



6 



Na 2 HP04 

 NaH 2 PC»4 



O.38I 



33 



0.70 



0.62 



45i 



No twitching. Death due to respir- 

 atory failure. 



8 



Na 2 SC»4 



O.25 1 



in 



1.29 



0.82 



480 



Twitching. 



115 (1697) 



The peptolytic enzymes of hemolytic streptococci ; methods. 



By RANDOLPH WEST and FRANKLIN A. STEVENS. 



[From the Medical Clinic of the Presbyterian Hospital, Columbia 

 University, New York City.] 



During the study of the virulence of hemolytic streptococci, 

 it has been necessary to understand the action of the cocci on 

 certain protein fractions. On account of the structure of these 

 bacteria, the methods required to obtain the active proteolytic 

 substance from the bacterial cell, and to accomplish the sterili- 

 zation of the solution containing the enzyme were at first 

 consuming and laborious. With the procedure outlined active 



1 These figures represent the average concentration. More dilute solutions were 

 employed at first and more concentrated ones later. See protocols in Greenwald, 

 Journal of Pharmacology and Experimental Therapeutics, 1918, xi, 281. 



