246 



Scientific Proceedings (115). 



The alterations in dimensional relations resultant from age, 

 from the incorporation of salts in concentrations within the 

 range of occurrence in the cell, and from hydration in various solu- 

 tions are to be included in the possibilities of conditions affecting 

 growth and cytological procedure. 



124 (1706) 



Is glycogen the source of acids developed in autolysis? 



By WITHROW MORSE. 



[From the School of Medicine, West Virginia University, 

 Morgantown.] 



In the following communication, an attempt is made to answer 

 the question whether glycogen contributes to the rise in acidity 

 in an autolyzing digest. 



Method. — Beef liver from the butcher was ground in sand, 

 diluted with Ringer's Solution to make a 20 per cent, digest by 

 weight and divided into two portions, I (control) and II, to which 

 one gram of glycogen obtained from liver was added for every 250 

 c.c. digest. In order to follow the rate and extent of digestion, 

 the following procedure was used: Fifty c.c. of the well-mixed 

 digest were transferred to a 100 c.c. volumetric flask and made up 

 to the mark with 5 per cent, trichloracetic acid. The mixture 

 was left until precipitation was completed (4 to 12 hrs.) and then 

 filtered. The nitrogen in 20 c.c. of the filtrate was then deter- 

 mined by Sorensen formol-titration. The reaction of medium 

 was studied by the following method : Fifty c.c. of the digest were 

 placed in fish-bladder dialyzing sacs and dialysis was made against 

 Ringer's Solution for 10 hrs. Hydrogen ion concentration was 

 then determined by the gas chain method, a Leeds and Northrup 

 Type "K" potentiometer, Weston standard cell and platinum 

 needle contact electrode being used. For the privilege of using 

 the Government apparatus in the West Virginia Experiment 

 Station, the writer thanks Professors Mcllvane and Morgan. 



In the following protocol, the averages of triplicate experiments 

 are given. The rate is given in cubic centimeters of decinormal 

 nitrogen, the hydrogen ion concentration in the Sorensen nomen- 

 clature (Ph) : 



