Multiple Infections with Treponema pallidum. 259 



stances of slight periostitis of the nasal bones. The infection 

 produced by the Nichols strain, which was isolated in 1912, has 

 been much more severe with pronounced primary lesions and the 

 frequent occurrence of extensive lesions of the bones, large cuta- 

 neous granulomata, lesions of the mucous membranes and of the 

 eyes. The time of occurrence of generalized lesions with both 

 strains is subject to wide variations but in general, is from 2 to 3 

 months after inoculation. In the vast majority of instances, 

 lesions of the periosteum and bone, skin, and eyes appear in the 

 order given. 



The rabbit was inoculated into the right testicle with Strain 

 III on December 2, 1920, with the production of an orchitis, which 

 pursued a normal course. On January 26, 1921, 55 days after 

 inoculation, the rabbit was reinoculated in the sheath with the 

 Nichols strain; at this time, resolution of the testicular lesions 

 was well advanced and the inguinal lymph nodes were barely 

 palpable. 



During the following week, a definite papule developed on the 

 sheath; the inguinal lymph nodes became enlarged and indurated 

 and activity of the orchitis was resumed. 19 days after reinocula- 

 tion, the lesion on the sheath was much larger than in any of the 

 controls and dark field examination showed actively motile 

 spirochete^. In addition, marked metastatic lesions were present 

 in the left testicle. 



3 weeks after reinoculation, or 2Y2 months after the primary 

 inoculation, there was a large actively growing chancre, an intense 

 metastatic orchitis, marked popliteal adenitis, a keratitis of the 

 right eye, and a number of bone lesions which resulted in extensive 

 necrosis of the nasal and tarsal bones and slight necrosis of the 

 distal end of the right ulna. The animal also showed pronounced 

 emaciation and weakness. During the next few weeks, the 

 keratitis completely disappeared and has not recurred and the 

 nasal lesions were also healed. The destruction of the tarsal 

 bones, however, was more extensive than has been observed in 

 any other animal infected with any strain of Treponema pallidum 

 and during the 2 months since the appearance of the lesions, very 

 little repair has been accomplished, a condition never before ob- 

 served. The chancre showed irregular periods of growth and 



