Anaphylactic Deaths in Guinea Pigs. 261 



tionship, therefore, these lesions are more closely related to the 

 second than to the first inoculation. 



Viewed in this light, it would be difficult to attribute the in- 

 fection produced in this animal to either of the strains of Trepo- 

 nema pallidum with which it was inoculated and although the out- 

 standing manifestations of disease conformed more to the general 

 character of the infection produced by the second than by the 

 first organism, it would seem to be more logical to regard the in- 

 fection as one produced by the combined action of the two or- 

 ganisms. These results are presented as a means of directing 

 attention to the possibilities of double or multiple infection where 

 appropriate conditions exist rather than to the probabilities of 

 their occurrence. 



133 (1715) 



Report on anaphylactic deaths in guinea pigs from intracutaneous 

 injection of small amounts of egg albumin. 



By HANS ZINSSER and S. T. WU. 



[From the Department of Bacteriology, College of Physicians and 

 Surgeons, Columbia University.] 



In view of the importance of the many problems arising in 

 regard to the relationship between intracutaneous skin reactions 

 and true anaphylaxis, the following laboratory "accident" would 

 seem well worth reporting. It seems especially interesting in 

 connection with the account of a human case of anaphylaxis fol- 

 lowing the intradermal injection of egg protein which was pub- 

 lished by Goestenberger and Davis, and which bears out certain 

 views concerning the relationship of bronchial musculature and 

 anaphylaxis brought out by Wells 1 in his recent critical articles in 

 the Physiological Reviews. The occurrence which is unique in 

 our experience with anaphylactic guinea pigs, was as follows: 



Six guinea pigs, 341, 349, 62, 568, 1122, and 1119, three males 

 and three females ranging in weight from 235 grams to 265 grams, 

 were treated on February 9 with intraperitoneal injections of 

 2 c.c. of a solution of crystallized egg albumin, representing about 



Wells, Physiological Reviews, 1921, i, 44. 



