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Scientific Proceedings (115). 



6.6 per cent, dry weight. The guinea pigs were used in experi- 

 ments in which parallelism between protein skin reactions in 

 hypersensitive animals and similar skin reactions carried out in 

 tuberculin sensitive animals, was being investigated. 



On February 12, 0.1 c.c. of a 1-4 dilution of 6.6 per cent, al- 

 bumin solution was injected intracutaneously into guinea pig 

 No. 349. No reaction resulted. 



On February 15, 0.1 c.c. of a 1-4 dilution of the original 6.6 

 per cent, egg albumin solution was injected intradermally in all 

 the pigs with three additional controls. The reactions were 

 watched from the time of injection for two hours, at very frequent 

 intervals, and after that, at longer intervals. At the end of 

 twenty hours, No. 1 122 showed what was considered a moderate 

 reaction, that is, an area of erythema, about one centimeter in 

 diameter, slightly elevated and slightly edematous. In No. 

 568 and No. 1 1 19 there was slight erythema, at the site of injection, 

 regarded as probably negative since similar erythema was present 

 in controls. 



On February 18, a second skin test was done on all the pigs 

 with 0.1 c.c. of crystallized egg albumin solution representing 

 about 11 per cent, estimated by dry weight, from a distilled water 

 solution. The injections at this time were carefully made by the 

 two writers together, and great care was taken to place the entire 

 amount intracutaneously, well-defined white wheals being formed 

 during the injection. Since no adverse symptoms were expected 

 immediately, all the pigs were put into a wire basket and carried 

 into the next room. Within a few minutes, No. 1 122, No. 349 and 

 No. 1 1 19 became sick, and No. 1122 and No. 349 were dead within 

 three minutes. It was unfortunate that the actual condition of 

 these pigs during these three minutes was not more carefully ob- 

 served, since they were dead by the time the basket was again 

 picked up, which was just three minutes after injection. From 

 the condition of No. 11 19, however, the symptoms can be in- 

 ferred, since this pig showed symptoms after three minutes, with 

 a tendency to fall down and very labored respirations, which con- 

 tinued for about an hour, gradually improving during this time. 

 This pig survived. Immediate autopsies done on the two other 

 pigs which died showed typical inflation of the lungs, the heart 



