Subcutaneous Tubes. 



281 



From such tubes, containing a small amount of aleuronat in 

 agar and filled up with Ringer's solution, leucocytes for phagocytic 

 experiments may be collected daily over a long period. During 

 the first day few leucocytes enter the tubes, but after 48 hours, 

 and subsequently, a 15-20 c.c. specimen of ground glass opacity 

 may be recovered from each tube. Practically all of these cells are 

 polymorphonuclear leucocytes and about 80 per cent, of them are 

 viable, as observed in a hanging drop in which the dead cells are 

 stained with aqueous carmine or trypan blue. Of the living cells, 

 15 per cent, or less will die during the 24 hours after removal, in- 

 dicating that 65-75 per cent, of the cells are in good condition, 

 an observation which is confirmed by their phagocytic activity. 



The introduction of bacteria into the tubes furnishes an op- 

 portunity for the observation of certain phases of the struggle 

 between invader and host. In general, the method seems appli- 

 cable to a variety of studies in chemotaxis and resistance to 

 invasion. 



142 (1724) 



Experiences with thyroidectomy and ligation of the thyroid arterie 

 in depancreatized dogs. 



By G. A. FRIEDMAN and J. GOTTESMAN. 



[From the Department of Clinical Pathology, College of Physicians 

 and Surgeons, Columbia University, New York.] 



The absence of glycosuria and the high sugar tolerance in 

 myxoedematous individuals led us to undertake the experiments 

 cited in this brief report. 



Dog No. 100, male, weight 14 kilo., partial pancreatectomy per- 

 formed January 12, 1921. Urine positive for sugar the following 

 day. It persisted in amounts of from 2 to 3 per cent, until Jan- 

 uary 19, when both inferior thyroid arteries were ligated. Glyco- 

 suria continued until January 26, 1921, when complete thyroi- 

 dectomy was done, sparing the parathyroids. Urine became 

 negative for sugar on the following day and continued so. On 

 February 5, 192 1, with blood sugar 95 mgms. an additional 3.4 



