Studies ix the Physiology of Vitamins. 291 



the desire to partake of food and the amount of vitamin-B which 

 is ingested. Yeast and tomato juice were used as sources of 

 vitamin-B in his experiments. The increase of appetite which 

 followed the administration of these products was believed to be 

 due to the vitamin-B contained therein since yeast appeared to be 

 less potent in this respect when autoclaved. This conclusion 

 is supported by the results obtained in our experiments in which 

 extracts of rice polishings, wheat embryo and navy bean were 

 tested for the property of promoting appetite in dogs which had 

 been fed on a diet lacking this dietary essential. The administra- 

 tion of any one of these preparations to such a dog was followed 

 by a recovery of appetite which lasted for varying periods. All 

 of these products were demonstrated to contain vitamin-B by 

 tests on polyneuritic animals (pigeons and dogs). The potency 

 of these products in promoting appetite seemed to parallel their 

 potency in relieving symptoms in polyneuritic animals and this 

 parallelism suggests that vitamin-B is the appetite-promoting 

 factor in the preparations used. 



149 (i73i) 



Further studies on the affinity of sheep-corpuscles 

 for anti-sheep hemolysin. 



By R. L. KAHN and D. S. LYON. 



[From the Bureau of Laboratories, Michigan Department 

 of Health, Lansing, Mich.] 



In previous studies 1 on the rate of absorption of anti-sheep 

 hemolysin by sheep corpuscles, it was shown that 0.05 c.c. of 

 packed sheep-cells added to a saline solution containing 400 

 units of hemolysin, will absorb as many as 390 units after 10 

 minutes extraction at room temperature. The hemolysin was 

 obtained by immunizing rabbits with sheep-cells in the usual 

 manner and a unit was taken to be the smallest quantity which 



1 Kahn, R. L., and Lyon, D. S., Proceed. Soc. of Amer. Bacter., Lansing Branch. 

 1920; Abst. of Bacter., 1921, v, 23. 



