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Scientific Proceedings (116). 



ridge which eventually pierces the cyst wall and forms the avenue 

 of escape of the small amcebulae. This may occur in the faeces at 

 the time of discharge or even later. 



The structure of the nucleus differs from that in E. coli. The 

 cysts are more difficult to stain. In the cysts the nuclear mem- 

 brane is lightly encrusted with granular chromatin, the central 

 karyosome is large, often asymmetrical or reniform, and in the 

 prophase is broken up into more or less distinct granules. It is 

 less often excentric than in E. coli. The interzonal area between 

 the karysome and nuclear wall stains lightly, if at all, in iron 

 haematoxylin. There are eight chromosomes at the metaphase, 

 while E. coli has six. An intradesmose forms within the nucleus 

 on the nuclear membrane at mitosis between the daughter centro- 

 somes which form deeply stained massive polar caps. 



The chromatoidal bodies are often massed in the early stages, 

 are splinter-like or thread-like, and pointed, and are rarely found 

 after the four-cell stage, except as rounding-up bodies. They 

 show a greater tendency to mass than in E. coli. 



The "glycogen" vacuole is present in the one to four-cell 

 stages. It is spheroidal, central, and lobed as it disappears. It 

 does not stain brown in iodine as in E. coli, though the cyst as a 

 whole stains yellow. It does not give the typical glycogen reac- 

 tion in Best's carmine. Otherwise in occurrence and behavior 

 it resembles the glycogen body of E. coli. 



As a result of bud formation and the discharge of amcebulae one 

 finds cysts with varying numbers of nuclei. We have seen from 

 three to twelve. The latter number appears to result from nuclear 

 multiplication during the period of escape of the amcebulae. We 

 have never seen this phenomenon of the ridge-like bud and the 

 successive discharge of amcebulae in E. coli. This budding of 

 amcebulae and the structure of the nucleus in the cysts distinguish 

 Councilmania from Endamceba and Endolimax. Councilmania 

 appears to be pathogenic, but more evidence is needed on this 

 point. It occurs also in carriers. 



