GRASSI 



263 



concerned the workmen and their families along 

 the Battipaglia-Reggio railway, 104 in all, including 

 33 children. The great majority of them had 

 suffered from malaria in the preceding year ; and 

 only 11, including 4 children, had never suffered 

 from it. Pending the arrival of the malarial 

 season, quinine was given to all who needed it. 

 The first Anopheles with its salivary glands infected 

 was found on 14th June. Twelve days later came 

 a case of malaria outside the "zone of experiment," 

 in a person who had never had malaria before. 

 The twelve days correspond to the incubation-period 

 after infection. Anopheles having come, and the 

 malarial season with him, the experiment was 

 begun. The houses were carefully protected 

 with wire netting, chimneys and all ; the siesta 

 was taken under wire netting ; the workmen, 

 if they were out in the evening or at night, wore 

 veils and gloves ; and Anopheles was to be killed 

 wherever it was found. Quinine was altogether 

 given up and forbidden, except for three workmen 

 who had escaped or evaded its use before June, and 

 had, indeed, never before been treated with quinine ; 

 one of them, moreover, had been sleeping outside 

 the zone of experiment in July. Except these three, 

 all the 104 and their doctors remained absolutely 

 free from malaria up to 16th September, the date of 

 Professor Grassi's report : — 



" Rightly to estimate the value of these facts, 

 it is necessary briefly to describe the surroundings 

 of the protected area. Towards the north, coming 

 from Battipaglia, three railway cottages are situated, 



