4M 



TR YPA NO SO MI DM 



pheral blood the centrosome inside the nucleus divides, and the new 

 centrosome, escaping from the nucleus, becomes the kinetonucleus, 

 and lies in the cytoplasm, which has now increased in amount. The 

 vesicle has gradually disappeared. A new fiagellum now appears, 

 and gradually the latent body becomes a trypanosome. The 

 rounded bodies with one or two nuclei, with or without the small 

 flagella, are probably identical with the forms found by Castellani 

 several years ago in the cerebro-spinal fluid of cases of sleeping 

 sickness, and described by him under the term ' amoeboid forms.' 



Fig. III. — Castellanella castellanii. Life-Cycle. 



Reproduction in the Fly.— The researches of Kleine, Taute, Bruce, 

 Hamerton, Bateman, and Mackie have shown that, after ingestion 

 at a correct period (as demonstrated by Miss Robertson), the try- 

 panosomes enter the stomach of the fly, and in twenty-four hours 

 lose their infectivity, as demonstrated by inoculation. According 

 to Miss Robertson, the trypanosomes multiply in the posterior part 

 of the mid-gut, and after the tenth or twelfth day give rise to long 

 forms which move forwards into the proventriculus between the 



