HMMOPROTEUS NOCTUM 



523 



2. Male Ookinete. — Smaller than the indifferent or female forms, with 

 cytoplasm almost hyaline, and much clearer than that of the indifferent form, 

 with granular material completely lacking, nucleus large, and rich in chromatin. 



3. Female Ookinete. — Cytoplasm fairly dense, with plenty of granules, with 

 a nucleus smaller than in the indifferent form. 



The Indifferent Ookinete. — The indifferent ookinete starts its development 

 by expelling for a second time a quantity of hsemozoin, while its kinetonucleus 

 unites with the trophonucleus, so that the nucleus now possesses eight com- 

 pound chromosomes. 



The centrosome becomes dumb-bell shaped, and forms an axial spindle, 

 around which the chromosomes are arranged. These now divide, and form a 

 diaster by the different portions passing to either end of the spindle, which is 

 heteropolar, the smaller half being kinetic in function. The nucleus now 

 divides into a larger portion, the trophonucleus, and a smaller, the kineto- 

 nucleus. The former enters on a resting stage, while the latter proceeds to 

 the periphery, and forming another axial spindle at right angles to the length 



On the left a small trypanosome is seen entering a red corpuscle in the 

 blood of the little owl, and in the corpuscle is seen the first endocellular 

 stage. The second corpuscle shows a more advanced endocellular stage 

 and the act of liberation into the liquor sanguinis. Farther to the right is 

 seen a medium-sized trypanosome. The third corpuscle shows the fully-grown 

 halteridial form, while on the extreme right a fully-grown trypanosome is 

 depicted. 



of the parasite, divides into two portions by its centrosome forming the axial 

 spindle and the chromosomes the two ends of a diaster. One of these daughter 

 kinetonuclei lying in the ectoplasm is attached to the other, lying in the endo- 

 plasm, by means of the axial spindle. The ectopias mic kinetonucleus forms 

 another spindle, with a longitudinal axis, which grows backwards along a 

 fold of the ectoplasm, expanding it as it goes, to the hinder end of the body. , 

 In this way an undulating mem_brane is formed, which, therefore, contains 

 eight chromosomes — -the myonemes — on each side of the folded ectoplasm, 

 while the axial spindle, becoming excentric, forms the fiagellum. At the 

 posterior end of the undulating membrane the fiagellum joins with the 

 chromosomes, and grows out of the body to form the free fiagellum, along 

 which the ectoplasm is drawn for a short distance. 



Of the two centrosomes of this spindle, the distal one disappears, while the 

 proximal one forms the blepharoplast at the root of the fiagellum. 



The endoplasmic daughter kinetonucleus becomes the kinetonucleus of the 

 trypanosome, and thus is formed the indifferent trypanosome, which now 



Fig. 184. — HcBMOproteus noctucs Celli and Sanfelice. 

 (After Schaudinn.) 



