iio6 



FUNGI IMPERFECTI 



Old cultures on glucose agar or on glycerinated glucose agar 

 were quite different in the two species. 



On carrot M. tozeuri attained a deeper brownish- yellow colour, 

 while in old cultures on this medium it produced spores in a manner 

 resembling an Oospora. 



Pinoy, in his remarks upon the mycology of these two species, 

 says that Brault's M. mycetomi very closely resembles that isolated 

 by NicoUe under the name of Oospora tozeuri. Its filaments are 

 2-8 microns in diameter, and do not possess apparatus for fructifica- 

 tion, reproducing by a breaking up of the hyphse of the thallus into 

 articles 5-10 microns in length, which divide into two spores. 

 These spores are of the same diameter as the hyphse from which 

 they arise, varying from 2-5 microns, while the membrane becomes 

 yellowish with age. In addition, chlamydospores can be observed 

 forming at the end of the filaments, more or less like favic nails. The 

 spores of M. tozeuri are smaller, but are formed in the same manner. 



On Sabouraud's gelatine M. mycetomi gives rise to black sclerotes 

 in the depth of the medium. These are very numerous, measure 

 i-i millimetre in diameter, and are composed of hyphal segments, 

 more or less cylindrical. Sometimes the sphere attains a diameter 

 of 10 microns, and usually contains only one nucleus; but, though 

 studied for a long time, these sclerotes were never observed to have 

 any higher form of fructification. In M. tozeuri it is very rare to 

 see the formation of sclerotes, which takes place on the surface of 

 the medium. 



On the bases of the researches on M. mycetomi and M. tozeuri, 

 Pinoy classifies the genus Madurella as follows : — 



A. Sclerotes O'5-i millimetre in diameter, formed in the depths of 



medium in cultures — Mycetomi. 



B. Sclerotes rarely produced, and then on the surface of the medium — 



Tozeuri. 



Madurella mycetomi (Laveran, 1902). 



Synonym, — Streptothrix mycetomi Laveran, 1902. 



Mycelium greyish white; when old, yellowish and darkening the 

 media in sugar cultures. Spores var3dng in dimension from 2-5 

 microns. Sclerotes black and sterile, with a diameter from 0-5-1 

 millimetre, formed in the depths of the medium in cultures. Can 

 invade the skin, bone, muscles, and connective tissue of man, 

 giving rise to black grains, which are small, hard, round, and more 

 or less warty, and which morphologically resemble the sclerotes 

 formed in the cultures. Up to the present the inoculation into 

 animals is negative. Very widely spread in Africa. Isolated by 

 Brault from a mycetoma with black grains in Algeria. 



Madurella tozeuri (NicoUe and Pinoy, 1908). 



Synonym. — Oospora tozeuri Nicolle and Pinoy, 1908. 

 Mycelium white, becoming yellowish with age and darkening the 

 medium in sugar cultures. Spores generally small, 2 microns or 



