ioi8 



ASCOMYCETES AND BASIDIOMYCETES 



recently four, further investigation having shown that the term he 

 used for one species (concentricum) covered more than one species. 



Endodermophyton tropicdle is very abundant in the lesions, 

 forming a felting of interlacing mycelial threads with mycelial 

 articles, regular in shape, rectangular or somewhat square-shaped, 

 and usually straight. If the liquor potassae be left to act some time, 

 the mycelial articles, which are of very variable length, and 2^ to 

 3 J [ji in breadth, will be seen to have a double contour. Aspergillus 



Fig. 514.- — ■ Endodermophyton Fig, 515. — Endodermophyton 

 tropicale Castellani: Glucose //'o^zca/g Castellani : Old Glu- 



Agar Culture. cose Agar Culture. 



fructifications, described by so many authors, when present, are due 

 to contaminations; they form no part of the fungus. Cultivation 

 of this fungus has been obtained by Castellani, using the method 

 mentioned in the paragraph above on the genus Endodermophyton. 



The principal cultural characters on solid media, when the growth 

 is fifteen to twenty-one days old, are as follows: — 



Glucose Agar (4 per cent.). — -Growth abundant; surface cerebriform or 

 crinkled. The growth and the medium show a sHght amber colour, which 

 later on may become of much deeper hue. Duvet as a rule absent, but in 

 old cultures which have been transplanted many times and are degenerating, 

 some very scarce, short, whitish duvet may appear. 



Sabouraud Agar. — Growth comparatively scanty, whitish-grey, mostly 

 submerged. The colonies are whitish, have generally a small central knob « 



