1022 ASCOMYCETES AND BASIDIOMYCETES 



Fig. 522. — Endodermophyion indicum Castel- 



LANI IN 'THE iSCALES. 



caps are used, both E. indicum and E. tropicale may assume a bright red 

 colour. If, however, subcultures are made from these, using tubes without 

 rubber caps, the fungi again show the characters given above. 



Hanging-Drop Cul- 

 tures. — Long mycelial 

 filaments are present. 

 No free spores are 

 seen ; reproduction is 

 apparently by sprouts 

 from the mycelium. 



Pathogenicity. — The 

 fungus is the cause of 

 a certain number of 

 cases of tinea imbri- 

 cata. The type of 

 the disease caused by 

 this fungus seems to 

 be more superficial 

 than that caused by 

 E. tropicale, but fur- 

 ther researches are 

 required on this sub- 

 ject. Castellani has succeeded in experimentally reproducing the 

 disease by inoculating coolies, "who had volunteered, with pure 

 cultures'of the fungus. The skin was first scarified with a sterile 

 knife; then a certain amount of a pure agar culture of E. indicum 

 was well rubbed in. After 

 fifteen to twenty-one days the 

 first signs of the eruption ap- 

 peared, and the typical patches 

 of tinea imbricata developed. 

 From the scales of the experi- 

 mental cases a fungus was 

 grown absolutely identical with 

 the strain of E. indicum with 

 which the individuals had been 

 inoculated. 



Endodermophyton castellanii 



Perry, 1907. 



Found by Castellani in cases 

 of tinea intersecta. In the scales 

 the mycelium is fairly abundant ; 

 no free spores are seen. Mycelial 



segments uniform, rather straight, and, provided the liquor potassae 

 be left to act for sufficient time, they often show a double contour. 

 Their breadth is 2 J to 31 [jl. Each mycelial segment has two 

 roundish refringent dots, one at each extremity. Has been 



Fig. 523. — Endodermophyton 

 castellanii Perry. 



