— 63- 



Distilled water 



Ammonium nitrate 



Potassium phosphate. . . . 



Magnesium sulphate 



Calcium chloride 



Dextrose 



Trace of ferrous sulphate 

 Agar 



500 cc. 

 •25 gr. 



. I 

 . I 



■05 



.005 " 



7 gms. 



The cultures were placed under bell jars in a north window and were exam- 

 ined almost daily. The spores continued to look green and healthy. After a 

 few days, probably through faulty sterilization or because of the dextrose, bac- 

 teria destroyed six out of ten plates. About four weeks later, the spores in the 

 good plates showed signs of swelling which is the first indication of spore germina- 

 tion. This was followed by a hyaline appearance at one end of the spore which 

 became egg-shaped because of the protruding germ tube through the ruptured 

 spore coat. 



Protenemal growth took place rapidly, the protenema being filamentous 

 alga-like in appearance. Transverse cross walls were cut off, the plate soon 

 becoming filled with this branching growth. In none of my cultures did I ob- 

 serve any oblique septa, which are indicative of rhizoid formation. The growth 

 was entirely apical, the lateral branches being formed behind septa. 



The buds were formed on the lateral branches. Walls intersecting each 

 other cut off portions of the ends of the filaments until a pear-shaped structure 

 resulted with cell division in three dimensions. From these buds the leafy 

 branch will originate. 



I made sections and then triple-stained portions of the cultures in order 

 to preserve the facts stated above: 



1. Spores before germination. 



2. Spores in the egg-shaped stage, showing the hyaline spot. 



3. Germ tube in the one- and two-celled stage. 



4. Buds in several stages. 



403 West 115TH St., New York City. 



HABITAT OF CEPHALOZIA FRANGISGI ON LONG ISLAND, N. Y. 



Fifteen miles west from the eastern extremity of the north prong of Long 

 Island is located Horton's Point, just north of the village of Southold. This is a 

 sandy beach, one to two hundred yards in latitude, extending two miles along 

 the coast of Long Island Sound. Inclosed on east and south by wooded hills 

 and shifting sand-dunes, on the west cut off by an inlet from the Sound. 



For a short distance back from the flood tide margin the beach is high and 

 dry and vegetated with Cladonia rangiferina, Panicum amarum, Prunus mari- 

 tima, Hudsonia tomentosa, Crysopsis, and clumps of shrub oak, Quercus stellata. 



Roy Latham 



