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INDIANA UNIVERSITY 



ting. Nor would the addition of calcium chloride solution cause 

 the blood to clot, either before or after the addition of disodium 

 phosphate. This would indicate that either the ferments or 

 some of the proteid elements of the blood had been affected by 

 the uranium and that its action is different from that of the 

 oxalates, for the careful addition of a slight excess of calcium 

 chloride causes oxalated blood to clot. Again the action of uranium 

 in preventing clot formation differs from that of the fluorides in 

 at least two particulars. The addition of a small amount' of throm- 

 bokinase derived by aqueous extraction from minced spleen and 

 lymphatic glands causes blood treated with potassium fluoride to 

 clot in almost the normal time. The addition of thrombokinase 

 is entirely without effect upon blood treated Avith uranium. Also, 

 if a small excess of calcium chloride be added cautiously to blood 

 treated with a fluoride solution clotting will occur. As stated above, 

 addition of calcium does not cause clotting in blood containing 

 uranium. It w^as supposed hy Rettger^° that the fluorides act by' 

 combining with the calcium to form calcium fluoride which in turn 

 is bound in some way to a portion of the protein. It may be that 

 uranium acts in some such manner as this, but it is to be noted 

 that blood treated with uranium behaves itself in an entirely differ- 

 ent manner toward calcium and thrombokinase from blood treated 

 with fluorides. It may be further mentioned that dialysis causes 

 fluoride blood to clot. I was unable to detect the formation of any 

 true clot in uranium blood after twenty-four hours of dialyzing in 

 running (tap) water. Uranium acts differently from magnesium 

 sulphate, inasmuch as the addition of thrombokinase causes clotting 

 in blood treated with that salt. And further, magnesium sulphate 

 blood may be made to clot by sufficient dilution with salt solution. 

 No amount of dilution will cause clotting in blood treated with 

 uranium. 



It might be questioned whether or not disodium phosphate 

 would precipitate out all the uranium when added to blood which 

 had been treated with that metal. If to a Ringer's or similar salt 

 solution to which a small amount (one-tenth volume of two per 

 cent solution) of sodium uranium tartrate has been added there 

 be further added a slightly larger quantity (one-quarter volume 

 of two per cent solution) of disodium hydrogen phosphate, a marked 

 precipitate will soon be produced. Mainly calcium phosphate comes 

 down at first, but after a little time it appears that the uranium 

 also is precipitated, for if blood be added to the clear filtrate, elot- 



i«Rettger, American Journal of Physiology, (1909) XXIV, 406. 



