1909.] HrBm-agglutinins, etc., in the Blood. 



77 



« 



No. of 

 cells 

 counted. 



No. of 

 bacilli 



in 

 cells. 



No. of 

 non- 



phago- 

 cytes. 



Experiment 1 — 



Untreated immune serum (A) + immune leucocytes (A) + B. coli. 

 Treated „ „ ,, >> „ 



60 

 50 



177 

 60 



2 

 10 



Experiment 2 — 



Untreated immune serum (B) + immune leucocytes (B) + B. coli. 

 Treated „ ,, ,, >< j. 



50 

 50 



167 

 5 



6 

 46 



Experiment 3 — 



Untreated normal serum (A) + normal leucocytes (A) + B. coli. 

 Treated „ „ „ „ 



50 

 50 



168 

 14 



5 

 38 



Experiment 4* — 



Untreated immimo serum (A) + normal leucocytes (A) + B. coli. 

 Treated ,, ,, „ 



50 

 50 



324 

 37 



4 

 29 



Experiment 5 — 



Untreated immune serum (B) + normal leucocytes (A) + B. coli. 

 Treated „ ,, „ „ ,, 



50 

 50 



233 

 59 



1 



ao 



Experiment 6 — 



Untreated normal serum (A) + immune leucocytes (A) + B. coli. 

 Treated „ „ „ „ 



50 

 50 



157 



59 



6 

 18 



* Active haemolysis was demonstrated. 



It is evident that these results do not depend on a hsemolysing agent, 

 because in five out of the six experiments neither agglutination nor haemolysis 

 occurred, and yet the degree of phagocytosis was very considerably reduced. 

 Attention has already been drawn to the reduced phagocytosis occurring as a 

 result of haemolysis, but from these experiments it has been shown that a 

 similar result may be obtained by thoroughly grinding red cells in serum from 

 the same individual and from other sources apart from true haemolysis. 



This action may depend upon the removal from the serum of incitor 

 substances, or by the addition to the serum of specific substances derived 

 from the red blood corpuscles, or lastly through non-specific substances 

 common to all red cells. Further experimental observations are necessary to 

 prove what these results are dependent upon. 



VOL. LXXXII. — B. 



H 



