1909.] Amakehe: A Disease of Calves in Uganda. 265 



The following chart shows the course of the temperature : — 



JJ&te 



/Uff. 1303 Sept. 







Z5 



Z£ 



zr\z8 







/ 



Z 





5 



6 



7 



a 



3 



10 



II 



IZ 



I5\H 



15 





IT 



IB 





ZO 



Zl 



zz 



104° 































































103° 















































t 



/ 













102° 



A 













t 



t 









t 









\ 







\ 



J" 



I 



J 



i 









u 



•- 





I 





101° 































































100° 





1 





























































Small 

 Firop. 































































+ 



Chart 9. — Experiment 659 represents the Temperature Curve of a Calf upon which 

 Infected Brown Adult Ticks had been fed. The minus and plus signs show the 

 absence or presence of the small rod and ring piroplasm in the blood. 



Remarks. — Twenty-one days after the infected brown adults had fed on this calf the 

 small piroplasm appeared in the blood. The tempei'ature curve is only slightly affected, 

 and the calf shows no symptoms of Amakebe. 



From the foregoing experiments it may be concluded, then, that the 

 appearance of Piroplasma higemimim or of the small rod and ring form of 

 piroplasm in the blood of a susceptible calf, whether introduced by the 

 injection of blood or, in the case of the latter, by the agency of the brown 

 tick, is not accompanied by the symptoms of Amakebe. It also is seen from 

 these experiments that the small rod and ring form is inoculable, is carried by 

 the brown tick, and the incubation period is long. This corresponds with the 

 description given by Dr. Theiler, Pretoria, of the piroplasm discovered by him 

 in the Transvaal, and named by him Piroplasma mutans. 



We may, therefore, consider that the two piroplasms which constantly 

 occiu' in the blood of Uganda cattle are those known as Piroplasma higemimim 

 and Piroplasma rmttans, and that neither is the cause of Amakebe. 



Is Amakebe Inomdable ? 



It has been shown that blood containing either Piroplasma higeminnm or 

 Piroplasma mutans if injected into susceptible cattle will give rise to these 

 diseases. Is it equally true that Amakebe is inoculable ? The following 

 experiments were carried out to obtain an answer to this question : — 



Experiment 1902. 



To ascertain if Blood taken from an Animal suffering from Amakebe and injected into a 

 susceptible Calf will give rise to the Disease. 



Feb. 22, 1909. — This calf was born last night. Placed in tick-free shed. 

 „ 26. — Injected with a 5 c.c. blood from calf, Experiment 430, suflfering from Amakebe. 



VOL. LXXXII. — B. 



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