316 Messrs. W. Cramer and H. Pringle. [Feb. 3, 



adeno-carcinoma (Tumour B obtained from the Imperial Cancer Research 

 Fund). Our results are given in Table I. 



Table I. — Giving the Absolute Amounts of Total Nitrogen expressed in Percentage of 

 Weight of Tissue, in the Various Tissues. 





Tumour. 



Heart. 



Muscle. 



Liver. 



Eadneys. 



Animals. 



Amount 

 of tissue 

 used in 

 grammes. 



N 



per- 

 centage. 



Amount 

 of tissue 

 used in 

 grammes. 



N 



per- 

 centage. 



Amount 

 of tissue 

 used in 

 grammes. 



N 



per- 

 centage. 



Amount 

 of tissue 

 used in 

 grammes. 



N 



per- 

 centage. 



Amount 

 of tissue 

 used in 

 grammes. 



N 



per- 

 centage. 



Tint T 



1 -095 

 1 '025 



2-31 

 2 -47 



0 -8040 



3-04 



0-3400 



3-74 



1 -0310 



3-04 







Eat II 



0 -2970 

 0 -3230 



2-83 

 2-77 



0-667 



3-23 



0 -4900 



3-63 



0 -5540 



3 -26 







Eat III 



No tu 



mour 



0-8400 



3-06 



0 -3850 



3-49 



0 -5720 



3 -08 







Mouse I ... 



0 -4134 

 0 -4390 



2-27 

 2 -23 







0 -3816 

 0 -4262 



2 -86 

 2-69 



0 -2900 

 0 -3170 



3-14 

 3-09 



0 -1780 



2 -98 



Mouse II ... 



Necrotic 

 part of 

 same 

 tumour 



0 -5898 

 0-7444 



! 0 -3193 

 r 0-3218 



2-25 

 2-33 



2 -.37 

 2 -39 











0 -2946 

 0 -3240 



2-75 

 2 -80 



0 -2426 



3-01 



Mouse III ... 



0 -7432 

 0-7700 



2-29 

 2-29 







0 -3073 

 0 -2435 



2-94 

 2 -82 



0 -3946 

 0 -3686 



3-19 

 3-19 



0 -1869 



3-29 



It will be seen that the amount of total N for the same tissues is 

 remarkably constant for different animals of the same species. In every 

 case the tumour tissue contains less nitrogen than the somatic tissues of the 

 host. This is true both for the sarcomatous and for the carcinomatous 

 tumours. In order to get an idea of the distribution of the nitrogenous 

 substances, we have divided them into (a) those whicli arc coagulated by 

 Ijoiling alcoliol, and (/;) those whicli are not coagulated. The latter do not 

 give the Ijiuret test. Tlicse two groups may be taken to correspond roughly 

 with native proteins on the one liand and simpler abiuret sid)stances on the 

 other. 



The determination of the relative amount's of coagulable and incoagulable 

 nitrogenous material was carried out by tlie method wliich we have used in 

 previous investigations.* This metliod we have found to give uniform and 

 relial)l(! nisults, and to be well ada])ted for the ])urposo of determining tlie 



* Harold I'ringle and \V. (Jramer, 'Journal of PhyHiology,' vol. 37, p. "2, 1908. 



