1910.] Contributions to the Biochemistry of Growth. 



317 



distribution of coagulable protein material in the various tissues. For this 

 investigation we used the tissues of Kats I, II, and III, as well as those of a 

 normal rat and of two rats, X and Y, of about the same weight, bearing very 

 large tumours also derived from the same transplantable rat sarcoma (J. K. S.). 

 The tumours of Rats X and Y were so large (30 grammes and 54 grammes 

 respectively), that they had begun to grow at the expense of the tissues of 

 the host. Eat Y was emaciated when killed. We also analysed the tissues 

 of two mice which had carcinomatous tumours of medium size. The 

 amount of tissue available from these latter being small, the tissues from the 

 two were pooled and analysed together, and so the figures are the average for 

 two. All the animals were kept on the same diet, viz., bread and milk. 



The following table gives the result of the analyses of various tissues, the 

 amounts of coagulable and incoagulable N are given in percentage of total N, 

 and the figures indicate therefore the relative proportions in which the two 

 groups of substances referred to above occur in the various tissues. 



Table 11. — Giving Proportion of Coagulable and Incoagulable Nitrogen 

 expressed in Percentage of Total Nitrogen present in the Various 

 Tissues. 





Tumour. 



Kidney. 



Liver. 



Muscle. 



Animal. 



Incoag. 



Coag. 



Incoag. 



Coag. 



Incoag. 



Coag. 



Incoag. 



Coag. 





N per- 



N per- 



N per- 



N per- 



N per- 



N per- 



N per- 



N per- 





centage. 



centage. 



centage. 



centage. 



centage. 



centage. 



centage. 



centage. 



Rat I 



19 -73 



80 -27 



13 -80 



86 -20 



9 -90 



90 -10 







Rat II 



20-64 



79 -36 



11 -85 



88 -15 



9-90 



90 -10- 







Rat III 



No tu 



mour 



14-10 



85 -90 



10 -40 



89 -60 







Rat X 



15-70 



84 -30 



17 -97 



82 03 



13 -83 



86 17 







Rat Y' 



18-74 



81 -26 



14 -37 



85 -63 



14 -20 



85 -80 







Normal Rat Z ... 



No tu 



mour 



14 -37 



85 -63 



11 -60 



88 -40 







2 mice (carci- 



23 -00 



77 -00 







17 -50 



82 -50 



16 -00 



84 -00 



noma) 



















Peferring to Table II, it will be seen that the analyses of the tissues of 

 Rats I and II which had small tumours agree together ; and that the 

 analyses of the somatic tissues of these also agree with those of Rats III 

 and Z, which were normal animals. Rats X and Y, which had large tumours, 

 begin to show anomalies, particularly the kidney of Rat X, where the pro- 

 portion of coagulable to incoagulable nitrogen deviates markedly from the 

 normal. 



There is a marked difference in the relative amount of coagulable nitrogen 

 in the different tissues examined, and it will be seen that, with the exception 



