318 



Messrs. W. Cramer and H. Priiigle. 



[Feb. 3, 



of Eat X, the tumour tissue in every case had a considerably smaller 

 percentage of coagulable nitrogen than the somatic tissues, liver, kidney, or 

 muscle (in the case of the mice). 



The average figures for the percentage of coagulable nitrogen are as 

 follows : — 





Tumour. 



Kidney. 



Liver. Muscle. 



Rats with tumours 



Mice with tumours 



81 -3 

 77 -0 



85 -50 

 85-76 



1 



88 -04 i 

 89-0 1 

 82 -5 84 -0 



It will be seen that the proportion between coagulable and incoagulable 

 nitrogen in tumour tissue and in somatic tissue is different in such a way 

 that in the case of the rats the relative amount of the coagulable nitrogen of 

 the tumour tissue is 4-2 per cent, less than in kidney, and 6'7 per cent, less 

 than in liver. In the case of the mice it is 5"5 per cent, less than in the 

 liver and 7 per cent, less than in muscle. 



In order to determine the absolute amounts of coagulable and incoagulable 

 nitrogen it is necessary to correlate these figures with the absolute amounts 

 of total nitrogen in the liver and the tumour* which are available in the 

 case of Rats I, II, and III from the protocol of the preceding paper.f In the 

 case of Rats X and Y, no determinations of the absolute nitrogen-content of 

 the various tissues were made, and as the stage of tumour growth was 

 different in the two groups, we have thought it better not to make the 

 results obtained from Rats 1 and 11 applicable to Ruts X and Y. In the 

 case of the mice, the small quantity of tissue available made it impossible 

 to make estimations of absolute amount of nitrogen and also of the coagulable 

 and incoagulable fractions in the same animal. We have therefore calculated 

 our results in the case of the mice by correlating our estimations of the total 

 nitrogen given in Table I with the figures giving the relative quantities of 

 coagulable and incoagulable nitrogen as given in Table II. 



Table III gives the absolute amount of co;igulabl(! and incoagulable, nitrogen 

 calculated from our data as above. 



The iigreenHiiit of the figures obtained for tlu; absolute amounts of coagu- 

 lable and incoagulable nitrogen in the somatic tissues ()l)tained in the series 

 of Rats I, ir, and III by individual estimations is striking, and affords 



* It wan iiiipoHHiblo to carry out (lu|)licate (letorininatioim of total nitrogen, coagulable 

 and incoagulable nitrogen, in the Hann; animal in the caHe of other tissues owing to the 

 small amount available. 



t W. Cramer and Tfarohl F'ringle, ' Itoy. Soc. Vyoc.,' unpra, p. HOT. 



