326 Messrs. A. Harden and W. J. Young. [Feb. 1 2, 



The following reactions for the sugars were then tried with equal volumes of each 

 solution : — 





1. 



2. 



3. 



b. Mohlisch's o-naphthol reaction 



c. SeliwanofF's resorcinol reaction 



for fructose 



1 



Intense violet coloration 



Slight reduction . . . 

 Violet coloration ... 

 Very faint reaction 



No reduction 

 No reaction 

 No reaction 



50 C.C. of each solution were mixed with equal quantities of phenylhydrazine 

 dissolved in acetic acid, and heated in boiling water for 1 hour. Osazones were forinetl 

 in Solutions 1 and 2, but not in 3. These osazones were filtered off, washed with water,, 

 dried, washed with ether, again dried and weighed. That from No. 1 weighed 

 0'09 gramme ; that from No. 2, O'Ol gramme. No. 1 melted at 166° — 168° and after 

 recrystallisation from boiling toluene, at 185° — 186°. The osazone from No. 2 melted at 

 196°— 198°. 



Hence a sugar has been produced by the action of zyniin freed froin, 

 coferment on the hexosephosphate, which has the characteristic reactions of 

 fructose, although the presence of other hexose.s is not exchided. 



A sugar of the same properties has ah-eady been shown to be formed wheiii 

 a hexosephosphate is hydrolysed by acids.* 



The decomposition products of hexosephosphate by enzymic and acid 

 hydrolysis appear, therefore, to be the same. 



(c) Fermentation by yeast of the sugar produced by the enzymic hydrolysis 

 of hexosephosphate. 



Experiment 9. — 20 grammes of zymin (Schroder) were washed as before and made to 

 100 c.c. with water. The following solutions were then incubated at 25° in presence of 

 toluene : — 



1. 50 c.c. of this suspension +40 c.c. of a solution of sodium hexosephosphate, equal to 

 27 c.c. of normal .solution, and equivalent, when completely fermented, to 302 c.c. of 

 carbon dioxide at N.T.P. 



2. 50 c.c. zymin suspension +40 c.c. water. 



3. 50 c.c. water + 40 c.c. sodium liexosepho9j)hate solution. 



No. 1 gave a total evohition of 107 c.c. of carbon dioxide, whilst Nos. 2 and 3 gave no 

 gas. At the end of three days the solutions were filtered, and treated as in Experiment iS. 

 The final solutions, free from hexosephosphate, were then incubated with a suspension uf 

 well-waslied living yeast, which had previously been auioformented for some days. 



* Young, 'Roy. Soc. Proc.,' B, 1909, vol. 8), p. 528. 



