402 



Dr. H. G, Chapman. On the Weight of [Apr, 21, 



Table III. 



No. of 

 tube. 



Antiserum. 



Amount 

 of 



antiserum. 



Weight of 

 dried 

 protein. 



Amount 

 of 

 saline 

 solution. 



Weight 

 of 



precipitate 





OCA II 1 1 1 J %J t t t t 





Til m.TYi a 

 lligrilis. 





lilgrjlls. 



1 



2 '5 



100 



■if) 



0 / 



2 





2 "5 



100 



50 



3 "5 



3 



TTpTl PflTcr-wliitp HQ 



egg WlXXLCj VC? 



2 '0 



134 



50 



8 '6 



4 



3 "0 



134 



50 



12 '5 



5 





4-0 



134 



50 



16 -7 



g 



Hi A1*CP BC.1.I1TY1 



xjLuroc Dcpuiii| oo... 





o\j 



0\J 



9 'Ci 

 ^ U 



7 





5-0 



200 



50 



4-0 



8 



Horse serum, 56... 



5-0 



100 



50 



10 -4 



9 





10 -0 



100 



50 



20 -0 



10 



Hen egg-white, 64 



I -0 



28 



50 



1 -4 



11 



2 0 



28 



50 



2 -2 



llB 







-1-140 





0-5 



12 



Hen egg-white, 64 



3 0 



28 



50 



3 -2 " 



12b 





-t- 56 





1 0 



13 



Hen egg-white, 64 



4-0 



28 



50 



3-6 ' 



13b 





+ 56 







2-4 



Weight 

 of precipitate 

 from 1 c.c. 

 antiserum. 



mgrms. 

 1 -5 



1 -4 

 4-3 

 4-2 

 4-2 

 0-8 

 0-8 



2 1 

 2 -0 

 1 -4 



1 -35 

 1 -4 

 1 -5 



amount of precipitate yielded by each antiserum is a fixed quantity for each 

 cubic centimetre of antiserum. It must be noted that the amount of saline 

 solution used as a diluent is the same throughout the series. In tubes 

 Nos. 1 and 2, 2'5 c.c. antiserum for horse serum interacted with 100 milli- 

 grammes dried horse serum and the duplicates agree well. In tubes 

 Nos. 3, 4, and 5, 2, 3, and 4 c.c. fowl's egg-white antiserum interacted with 

 100 milligrammes dried egg-white, yielding 8'6, 12"5, and IG'T milligrammes 

 precipitate respectively. Calculating the amount for each cubic centimetre anti- 

 serum it is found to be about 4 2 milligrammes in each case. In tubes Nos. 6 

 and 7, 2"5 c.c. antiserum for liorse serum reacted with 50 milligrammes 

 horse serum, and 5 c.c. antiserum reacted with 200 milligrammes horse 

 serum. The amount of precipitate in tube No. 7 was double that in tube 

 No. 6. In tubes Nos. 8 and 9, 5 c.c. and 10 c.c. antiserum each interacted 

 with 100 milligrammes dried egg-white and the weights of the precipitates 

 agree sufliciently for each cubic centimetre. In tul)es Nos. 10, 11, ]2, and 

 13, 1, 2, 3, and 4 c.c. antiserum for fowl's egg-white each reacted with 

 28 milligrammes dried egg-white. After -48 hours the superfluids were 

 removed and a quantity of dri(Ml egg-wliite dissolved in saline solution 

 added to each superlluid. No further precipitate formed in tube No. 10, but 

 precipitates formed in tubes Nos. Hit, 12b, and 13ii. Here, again, there is 

 fair agreement in the amount of precipitate finally obtained from each cubic 

 centimetre of antiserum. 



