1910.] Injiuence of Bacterial Endotoxins on Phagocytosis. 409 



Experiment 3. 



A, Serum saline + leucocytes + B. xiroteus — 



50 cells contained 474 bacilli ; non-phagocytic cells, 0. 



B. Serum proteus extract + leucocytes + B. proteus — 



50 cells contained 33 bacilli ; non-phagocytic cells, 24. 



The following experiments demonstrate the completely specific action of the 

 endotoxin : — 



No. of 

 bacteria in 

 50 cells. 



No. of 

 non-pliagocytio 

 cells. 



Experiment 1. 



Serum saline + leucocytes + B. paratyphosus B 



„ + „ + B. Danysz 



Serum paratyphosus B. extract + leucocytes 



+ B. paratyphosus B. 

 „ „ „ + leucocytes 



+ B. Danysz 



Experiment 2. 



Serum saline + leucocytes + B. Achard 



„ + „ + B. Danysz 



Serum B. Danysz extract + leucocytes + 5. Achard 



„ II j> + 1. + -S. Danysz 



Serum B. Achard extract + leucocytes + -B. Achard 



„ !. )> + » + J^^nysz 



Serum 5. eo?i extract + leucocytes + J. ^cAarrf ... 



,, „ + ,, +B. Danusz ... 



Experiment 3. 



A. Serum saline + leucocytes + B. ■pyocyaneus 



„ + ,, + M. aureus 



B. Serum B. pyocyaneus extract + leucocytes + B. pyocyaneus 



„ „ + ,, + M. aureus 



), >■ + )■ + B. pyocyaneus 

 ,, ,, + ,, + M. aureus 



C. Serum 31. aureus -e^tr&ct + leucocytes + B. pyocyaneus 



„ „ + ,, + 3£. aureus 



„ ,, + ,, + B. pyocyaneus 



,, + ,, + M. aureus 



equal 

 " vols. 



192 

 62 



15 

 89 



249 

 268 

 102 

 25 

 10 

 123 

 156 

 183 



234 

 99 

 7 



56 

 7 



36 

 236 



36 

 167 



53 



0 

 16 



39 

 13 



1 

 1 

 11 



33 

 42 

 11 



2 

 3 



0 

 20 

 44 

 24 

 44 

 36 



4 

 38 



6 

 28 



In the third experiment of this series, the specific nature of the endotoxic 

 action is clearly shown when the leucocytes and equal volumes of cocci and 

 bacilli are mixed together. 



"We may remark that the degree of phagocytosis in all our experiments is 

 estimated by the number of organisms engulfed in a given number of cells, but 



