500 Col. Sir D. Bruce and others. Mechanical [June 22, 



Experiment 1543. Interrupted Feeding. 



Laboratory -bred Glossina palpalis and Trypanosoma gambiense. Infected monkey to 



healthy goat. 



Date. 



Day. 



Feedings from infected monkey to 

 healthy goat. 



Remarks. 



1909. 



Aug. 28 



„ 29—30 , . 

 Sept. 9 



1 



2—12 



20 alternate feeds. 

 25 



Continued daily for 12 days. 



50 flie8 used. 



Result. — Positive. The goat, Experiment 1495, was examined daily for trypanosomes 

 until October 16, but none was seen. On October 1, 21 days after the last feed of 

 infected Glossina palpalis, 4 c.c. of the blood of this goat were injected into monkey, 

 Experiment 1777. This monkey showed trypanosomes on October 16, proving that the 

 goat. Experiment 1495, had become infected from the monkey by interrupted feedings 

 though trypanosomes failed to appear in its blood. 



Experiment 1565. Interrupted Feeding. 



Laboratory-bred Glossina palpalis and Trypanosoma gambiense. Infected ox to monkey. 



Date. 



Day. 



Interrupted feeding on infected ox 

 and healthy monkey. 



Bemarks. 



1909. 

 Aug. 29— Sept. 9 



1—11 



25 passages to each animal, the whole 

 lasting about half an hour. This 

 continued daily for 11 days. 



30 flies used. The mon- 

 key, examined for 

 30 days, failed to 

 show trypanosomes. 



Result. — Negative. Blood of ox injected into monkey on August 28. This monkey 

 became infected with Trypanosoma gambiense six days later. The negative result may 

 be due to the small numbers of trypanosomes found at any time in oxen. 



Experiment 1705. Interrupted Feeding. 



Laboratory-bred Glossina palpalis and Trypanosoma gambiense. Infected monkey 



to fowl. 



Date. 



Day. 



Interrupted feeding on infected 

 monkey and healthy fow). 



Bemarks. 



1909. 

 Sept. 28— Oct. 4 



1-6 



30 passages from monkey to fowl, and 

 continued daily for (i days. 



i 



60 flics used. 



Result. — Negative. October 7, 1 c.c. of blood of fowl injected into healthy monkey. 

 November 2, 2 c.c. of blood of fowl injected into healthy monkey. Both monkeys 

 remained healthy. 



