1910.] 



AutotoxcBYiiia and Infection. 



539 



Chart 10. 

 A. 



1 











1 1 







1 P 













(A 

 1 



















17 





24 













9 



12 



6 



9 



3 





9 





6 



9 



















9 





9 





















































































































A 



B 











































































































































































F 















































































HOUR 

 GRM5. 

 620 

 6 I 0 

 600 

 590 

 580 

 5 70 



A.B. C,D E.F = 100. 0 c.c. m divided doses in33 hours 



B. 



A. 8 , C , D. = 70.0 c.c. in divided doses in 33 hours 



C. On the Antitri/ptic Values of the Serum. 

 (a) Rabbits. 



Twenty cubic centimetres of water were injected subcutaneously, in four 

 divided doses, into a healthy rabbit. The animal was bled immediately before 

 the first injection, and 60 minutes after the last injection, to the extent of 

 5 c.c. on both occasions. A control animal was bled at the same times, but 

 was not injected. Estimation of the antitryptic values of all four serums was 

 made the following morning. In order to ensure accuracy of observation in 

 estimation of the antitryptic values of the serums two methods were 

 employed : (1) The chemical method of Sorensen^ for estimating the 

 velocity of action of a trypsin-casein mixture (by an adaptation of which 

 I had shown that it was possible^ to determine variations in the antitryptic 

 values of the serum of subjects of malignant disease with sufficient 

 accuracy to aid materially in diagnosis). (2) The viscosity method introduced 

 by Spriggs for estimating the velocity of a trypsin digestion adapted by 

 Golla^ for determining antitryptic values of serum in normal and pathological 



