564 Miss Fraser and Mr. Gardner. Origin and [June 17, 



ether extract, which gave 0'1218 gramme compound = 0"0296 gramme 

 cholesterol, a percentage of 0"0575 free cholesterol in the blood ; and 



(b) By saponification 44'56 grammes of blood yielded 0'1074 gramme ether 

 extract, which gave 0'1642 gramme compound = 0"0399 gramme cholesterol, a 

 percentage of 0'089o total cholesterol and 0'0320 per cent, ester cholesterol. 



Babbit 3. — Fed for five days on extracted bran. Weights of rabbit 

 during experiment = 3"3, 3"2, and 3"2 kilogrammes. Total weight of blood 

 = 72-3301 grammes. 



(a) By direct precipitation 31'8071 grammes of blood yielded 0'0838 gramme 

 ether extract, which gave 0'0594 gramme compound = 0'0144 gramme 

 cholesterol, a percentage of 0"0454 free cholesterol ; and 



(b) By saponification 40"523 grammes of blood yielded 0'0702 gramme ether 

 extract, which gave 0"1126 gramme compound = 0'0274 gramme cholesterol, 

 a percentage of 0'0675 total cholesterol and 0'0221 ester cholesterol by 

 difference. 



Babbit 4. — Fed for five days on extracted bran. Weights of rabbit taken 

 periodically during experiment = 2, '1'9, 2 kilogrammes. Total weight of 

 blood = 43"4464 grammes. 



By saponification 22'3374 grammes yielded 0'0518 gramme ether extrac-t, 

 which gave 0'0622 gramme compound = 0"0151 gramme cholesterol, a 

 percentage of 0'0677 total cholesterol. 



The same conditions were carried out with regard to another pair of 

 rabbits, one being fed on extracted bran, the other on extracted bran and 

 measured quantities of cholesterol. 



Babbit 5. — Fed on extracted bran for eight days. Weights of rabbit taken 

 periodically = 2-3, 2-3, 2-2, 215, 2-2, 2-2, 2-2 kilogrammes. Total weight of 

 blood = 65'54 grammes. 



(a) By direct precipitation 33"82 grammes of blood yielded 0'0738 gramme 

 ether extract, which gave 0'0G3O gramme compound = 0*0153 gramme 

 cholesterol, a percentage of 0'0453 free cholesterol in the blood ; and 



(b) V,y saponification 31"72 grammes of blood yielded 0'0868 gramme ether 

 extract, whicli gave 0'0722 gramme compound = 0"0175 gramme cholesterol, 

 a percentage of 0"0553 total cholesterol and O'Ol ester cholesterol. 



Babbit 6. — Fed on extracted bran plus cholesterol for eight days. No 

 cholesterol i'or first two days, ^ gramme for three days, and ^ gramme for 

 last three days. Total weiglit of cholesterol = 2^ grammes. Weights of 

 rabbit = 2-8, 27, 2-G5, 2-G, 2-G, 2-G5, 27 kilogrammes. Total weight of 

 blood = G8-21 grammes. liabbit killed six hours after administration of 

 cholesterol. 



{(i) By direct precipitation 37 00 grammes of blood gave 0'0996 gramme 



