1910.] Destiny of Cholesterol in the Animal Organism. 565 



•compound = 0 0242 gramme cholesterol, a percentage of 0 0653 gramme 

 of free cholesterol in the blood. 



(ft) By saponification 3i'12 grammes of blood gave 0*1079 gramme 

 compound = 0'0262 gramme cholesterol, a percentage of 0 '08425 total 

 cholesterol and 0'019 ester cholesterol. 



Table V. — Percentage of Cholesterol in Blood. 



Rabbit. 



Total. 



Free. 



Ester 

 (by difference). 



1. 



Fed on extracted bran and 



cholesterol 



0 -0877 



0 -0492 



0 -0386 





(2 grammes) 











2. 



Fed on extracted bran and 



cholesterol 



0 -0895 



0 -0575 



0 -0320 





(2 grammes) 











3. 







0 -0675 



0 -0454 



0 -0221 



4. 







0 -0677 







5. 



Fed on extracted bran and 





0 0553 



0 -0453 



0 -0100 



6. 



cholesterol 



0 -0843 



0 0653 



0 -0190 





(2t grammes) 











We found in our previous experiments on haemolysis,* that when phytosterol 

 ■was given with the food the anti-heemolytic power of the serum was increased, 

 as in the case of cholesterol. We therefore made a comparison of the blood 

 of rabbits fed with ordinary bran, which contains a phytosterol, and the 

 blood of rabbits fed on extracted bran, with regard to the cholesterol and 

 cholesterol ester content. 



Eabhit 7. — Fed on ordinary bran for nine days. Weight of animal during 

 experiment = 2-0, 2-0, 21, 21, 21, 21, 2-0, 21 kilogrammes. Total weight of 

 blood = 61 •9405 grammes. 



{a) By direct precipitation 31"5175 grammes blood gave 0"084 gramme 

 compound = 0"0204 gramme cholesterol, a percentage of 0"0648 free 

 cholesterol in the blood ; and 



Q)) By saponification 30*423 grammes of blood gave 0"0962 gramme 

 compound = 00234 gramme cholesterol, a percentage of 00768 total 

 cholesterol, 0 0121 ester cholesterol. 



Babbit 8. — Fed on ordinary bran for nine days. Weights of animal = 2-6, 

 2-7, 27, 2-7, 2-7, 2-7, 27 kilogrammes. Weight of blood = 71-2945 grammes. 



(a) By direct precipitation 35"3045 grammes of blood gave 01023 gramme 

 compound = 0-0249 gramme cholesterol, a percentage in the blood of 

 0*0741 free cholesterol ; and 



(b) By saponification of ether washings from (a) 35*3045 grammes of blood 



* Ibid., p. 242. 



2 u 2 



