1910.] Quantitative Estimation of Hydrocyanic Acid, etc. 579 



10 c.c. of blood diluted with water to 75 c.c. plus a little tartaric acid, distilled, and the 

 distillate received in three successive portions, each 15 c.c, in 50 c.c. of picrate of soda 

 solution. Next day the three solutions were as follows : — 



1. The first distillate (65 c.c.) had a red colour = T 8, indicating 0 000520 gramme HCN. 



2. „ second „ (65 c.c.) „ „ = T 4, „ 0-000260 „ „ 



3. „ third „ (65 c.c.) „ „ = T O'S, „ 0-000052 „ „ 



Another 10 c.c. of blood was left freely exposed to air for 48 hours and then distilled 

 in two successive portions : — 



4. The first portion was of a colour = T 1-5, indicating 0 000090 gramme HCN. 



5. „ second „ „ = TO-75, „ 0-000045 „ „ 



Experiment 2. — Kitten : 0-6 kilo. ; chloroform ; 10 c.c. of 1 per cent, solution of HCN 

 poured into the stomach. Immediate death. 10 c.c. of defibrinated blood, diluted with 

 water and distilled into 50 c.c. of sodium picrate, gave T 5 (i.e. 0-000300 gramme HCN). 



Experiment 3. — Cat ; 2 kilos. ; chloroform ; 10 c.c. of 1 per cent. HCN into stomach. 

 .Immediate death. Distillation into 25 c.c. picrate. 



Blood 



Plasma 



Corpuscles ... 



First day distillate — 



Gramme HCN per 

 10 grammes tissue. 



1. From 10 c.c. of defibrinated blood diluted to 60 c.c. T 9 in 31 c.c. 0-000279. 



2. „ 10 c.c. of plasma T 8 in 29 c.c. 0-000252. 



3. „ 5 c.c. of corpuscles T 5 in 30 c.c. 0-000300. 



The diflFerence between blood, plasma, and corpuscles as regards HCN is not very 



marked. 



Second day distillate, the blood having been left exposed to air — 



4. From 10 c.c. plasma T 0-75X32. 0-000024. 



5. „ 5 c.c. corpuscles T3-5X30. 0-000210. 



Plasma 



Corpuscles 



Experiment 4. — A control experiment. Cat ; 3 kilos. ; killed by chloroform. Distillates 

 into 25 c.c. picrate from 10-c.c. of blood and from 10 grammes of brain. No change of 

 colour in the picrate in either case. 



Experiment 5. — Cat ; 3 kilos. ; chloroform ; 10 c.c. of 1 per cent. HCN poured into the 

 .stomach ; immediate death ; distillates taken at once. 



e 'OO 2.00 300 4^0 



Plasma 



Curpuscles ... 

 Brain 



Distillate 1 from 10 c.c. plasma gave T 7-5 in 32 c.c. 0-000240. 



„ 2 „ 10 c.c. corpuscles „ T 10 in 32 c.c. 0 000320. 



„ 3 „ 10 gr. brain „ T 15 in 30 c.c. 0-000450. 



vVOL. LXXXII. — B. 2 X 



