580 



Dr. A. D. Waller. New Method for the [June 17, 



The remarkable feature here is the large amount of HON distilled from the brain. 

 Three days later a distillate of the second half of the brain, weighing 10 grammes, gave 

 0-000045. 



Experiment 6. — Cat ; 2-4 kilos. ; chloroform ; 10 c.c. of 1 per cent. HCN injected per 

 jugidam ; immediate death ; brain removed at once ; blood defibrinated and centrif uged 

 at once. Materials kept in bottles till next day. 



1 090 ' 5 go 



Brain 



Plasma 



Corpuscles 



Sixteen hours later — 



Distillate 1 from 10 grammes brain gave T 60 in 33 c.c. 

 „ 2 „ 10 c.c. plasma „ T 18 in 32 c.c. 



„ 3 „ 10 c.c. corpuscles „ T 30 in 33 c.c. 



0-001980. 

 0-000576. 

 0-000990. 



So far these are the highest numbers obtained ; the exit tube from the still dipped into 

 the picrate solution and there was no appreciable loss of HCN vapour. The colour of the 

 brain distillate was that of port wine ; the calculated amount of HCN was nearly 

 2 milligrammes per' 10 grammes {i.e. 480 milligrammes per 2-4 kilos, if the poison had 

 been equally distributed over the body of the cat ; the actual injection had been 

 100 milligrammes or 42 milligrammes per kilo. The amount found in the brain was 

 198 milligrammes per kilo.). 



Three days later — 



Distillate 4 from 10 c.c. blood gave T 10 in 31 c.c. 0-000310. 

 „ 5 „ 10 grammes brain „ T 30 in 30 c.c. 0-000900. 



Six days later — 



Distillate 6 from 10 grammes brain gave T 10 in 30 c.c. 0 000300. 

 „ 6a „ 10 „ „ „ T 1 in 30 c.c. 0-000030. 



Experiment 7. — Cat ; 2-5 kilos. ; chloroform ; three intravenous injections of 10 c.c. 

 1 per cent, solution of amygdalin in the course of half -an -hour (=100 milligrammes = 

 5-3 milligrammes HCN), immediately followed by 10 c.c. of 0-1 per cent, solution of 

 emulsin. No marked effects. Finally, two injections of 10 c.c. of a freshly made mixture 

 of equal volumes of 1 per cent, amygdalin and 1 per cent, emulsin. Death at the second 

 injection. 



. The distillate into picrate of 10 c.c. of defibrinated blood gave T 1-2. 0-000072. 



Experiment 8. — Cat ; 2*4 kilos. ; chloroform, 2 per cent. ; 10 c.c. of 1 per cent. HCN 

 solution (0-1 gramme) poured into stomach ; immediate death. J.)istillates taken from 

 10 grammes. 



loo zoo 



Blood... 

 Brain ... 

 Heart... 



Muscle 



Distillate 1. Blood T 8 in 30 c.c. 



„ 2. Hind brain T 1 in 30 c.c. x 2. 



„ .3. Fore brain T 1 25 in 30 c.c. X 2. 



„ 4. Heart muscle ... T 6 in 30 c.c. 



„ 5. ( Jlutcal iiiUHcle ... 'I' 0 ill 2H c.c. 



0-000240. 

 0-000060. 

 0-000075. 

 0-0()01H(). 

 Nil. 



