1910.] Quantitative Estimation of Hydrocyanic Acid, etc. 581 



The remarkable point in this experiment was the presence of HON in cardiac muscle, 

 its absence from skeletal muscle. Subsequent experiments show that this difference is a 

 characteristic feature. 



Experiment 9.— Cat ; 2-7 kilos. ; chloroform, 2 per cent. ; 10 c.c. of 2 per 10,000 HCN 

 solution (0'002j injected by the femoral vein lowered the blood pressure; then 10 c.c. 

 1 per 1000 (0-01) killed the animal. 



Distillate 1. Blood T 2 in 30 c.c. 0-000060 gramme HCN. 



„ 2. Brain T 2 in 29 c.c. 0-000058 „ „ 



„ 3. Heart T 3-5 in 31 c.c. 0-000108 „ „ 



Experiment 10. — Cat ; 3 kilos. ; chloroform, 2 per cent. ; 10 c.c. of 0-5 per cent. HCN 

 solution (0-05 gramme) injected by the femoral vein. Distillates from 10 grammes. 



Blood ... 

 Brain ... 

 Heart... 

 Muscle 



iao 1000 



Distillate 1. Blood T7 x30( + T3X30). 



„ 2. Brain T 16 x 31 ( +T 3 X32). 



„ 3. Heart ... T 25 x 31 c.c. 



4. Muscle ... T 3-5x31 



0-000300 gramme HCN. 

 0-000592 „ „ 

 0-000775 „ 

 0-000108 



Note. — The heart weighed 12-3 grammes. The brain weighed 23-2 grammes. 



Experiment 11. — Cat ; 2-2 kilos. ; chloroform, 2 per cent. Death by injection of 1 c.c. 

 of 0-5 per cent, solution of NaCN(= 5 milligrammes NaCN = 2-6 milligrammes CN). 

 Distillates of 10 grammes into 10 c.c. picrate. 



Blood... 



Brain ... 

 Heart... 

 Muscle 



Distillate 1. Blood T 1-5x20. 



„ 2. Brain T 4 5X20. 



„ 3. Brain, (2nd distillate) ... T0x20. 



Distillate 4. Heart T 4-5 x 20. 



„ 5. Muscle T0x21. 



0-000030 gramme HCN. 

 0-000090 „ „ 

 Nil. 



0-000090 „ „ 

 Nil. 



2x2 



