1910.] Quantitative Estimation of Hydrocyanic Acid, etc. 583 



Distillate 1. Blood T 2-5x21. 



„ 2. Brain T 4-5X 20 x 10/8. 



„ 3. Heart T 2-0x20. 



„ 4. Muscle Nil. 



„ 5. Brain (2nd distillate) ... Nil. 



0 •000052 gramme HCN. 

 0-000112 „ „ 

 0-000040 „ „ 

 Nil. 

 Nil. 



Experiment 13 (June 9, control). — Cat ; 2-5 kilos. ; CHCI3, 2 per cent, for 68 minutes, 

 then at 5 per cent, for 6 minutes. Death. Distillates (20 c.c.) of 10 grammes of blood, 

 brain, and heart into 10 c.c. picrate gave TO, i.e. no HCN. 



Experiment 14 (June 9, control). — Cat ; 2-6 kilos. ; ether, 12 per cent, for 23 minutes, 

 then raised to 20 per cent, for 13 minutes. Death by pithing. Distillates as above, of 

 blood, brain, and heart, gave no colour change, i.e. no HCN. 



Experiinent 15 (June 10), — Cat ; 2-8 kilos. ; ether at 20 per cent, for 13 minutes. 

 Eespiration stopped, but immediately restored by artificial means. Ether at 12 per cent. 

 Thirty-one minutes later, death by intravenous injection of 5 c.c. of 1 per 1000 HCN 

 ( = 5 milligrammes). Distillates as usual. 



Blood 



Brain 



Heart 



Medulla.. 

 Sp. cord.. 

 Muscle .. 



Distillate 1. Blood T5x22c.a 0-000110. 



„ 2. Brain T 6*5x22 c.c. 0-000143. 



„ 3. Heart T 7 x 25 c.c. 0-000175. 



„ 4. Medulla T6 x 23 c.c. 0-000138. 



„ 5. Spinal cord ... T3 x 21 c.c. 0-000063. 



„ 6. Muscle TO X 21 c.c. Nil. 



Experiment 16. — Cat ; 2-6 kilos. 

 HCN on blotting paper. 



Death in one minute by inhalation of 1 per cent. 



1. Blood T 0-75x20 c.c. 0-000015. 



2. Brain T 0-5 x 20 c.c. 0-000010. 



3. Heart T 0-75x20 c.c. 0-000015. 



4. Muscle TO X 20 c.c. Nil. 



Note. — No estimate is possible of the amount of HCN actually inspired. Judging 

 from the amounts found as compared with those found in the previous experiment, the 

 amount inspired must have been much below 5 milligrammes. 



Experiment 17 (June 11). — Cat ; 2-8 kilos. ; ether. Death by 5 c.c. of 1 per cent. HCN 

 poiired into stomach. Body left untouched for 50 hours, when the usual distillates were 

 taken, with these results : — 



loo loo 300 4.00 Joo 



Blood 



Brain 



Heart 



Muscle 



Stomach ... 

 Duodenum 



