610 Prof. Macallum. Inorgcmic Composition of the [June 23, 



the lime as oxalate. The magnesium in the filtrate from this was precipitated 

 on the addition of more ammonium phosphate and of strong ammonia, and 

 the filtered fluid evaporated to dryness and heated to expel the acetic acid 

 and ammoniiim acetate. To the residue, dissolved in a small quantity of 

 dilute hydrochloric acid, saturated baryta water was added to remove 

 the sulphuric and phosphoric acids, the excess of the baryta was pre- 

 cipitated with ammonium carbonate, and the filtrate from this was treated in 

 the way described above, to determine the sodium and potassium. 



III. — The Results. 



A. The Blood Serum of Limulus. — The blood of Liinuhis was coagulated, 

 and contained a large quantity of fibrin, which was removed by straining 

 through fine muslin cloth. The serum was deep azure-blue in colour, 

 and had a specific gravity of 1-03847. Its A was -2-04° C. The A 

 of the salts in the blood was — 1'875° C. The total salts amounted to 

 2'982 per cent. The percentages of solids in four different estimations were 

 7-960, 7-822, 7-942, and 8-056. The different results in the four estimations 

 were due to differences in the time during which the residue was dried, first 

 in a steam-heated oven, and then at 110° C. The average per cent, of these 

 determinations was 7-945. The analyses of the ash gave the following : — 





I. 



II. 



III. 



Mean. 



Na 



0 -8944 



0 -8864 



0 -8848 



0 -8885 



K 



0 -04951 



0 -0500 



0 -05017 



0 -04989 



Ca 



0 •03614 



0 -03621 



0 -03604 



0 03618 



Mg 



0 -0995 



0 0996 





0 -09955 



CI 



1 -0526 



1 -669 





1 -6608 



SO3 



0 -11718 



0 -11976 





0 -11847 



Cu 



0 -00742 



0-00773 



0 -00806 



0 -00773 











2 -86107 



B. The Blood Serum of the Lobster. — The blood of the lobster was rich in 

 fibrin, and was of a light blue colour. The specific gravity of the serum was 

 1-0337. The solids in two estimations were 8 ;)54 and 8-351 i)er cent., and 

 the mean 8-3525 per cent. The total salts in two estimations were 2-855 

 and 2-849 per cent., the mean 2-852 p<!r cent. Tlic A due to the salts 

 ascertained from these two determinations wen; —1-73° and —1-735°. The A 

 of tlie serum in two dirierent detci-minationis was —1-78° C. The sea water 

 from the bottom of the l)ay (at St. Andrews, New J^runswick), where tlie 

 lobsters were caught, gave a A of —1-76^', that of tlu; surface at tlie same 

 ])oiMt — 1-6;!5'^. 'I'lu! analyses of the ash gave': 



