260 



Inoculation for Pleuro- Pneumonia in Cattle. 



but thin. The swelling of the surrounding parts is augmented^ 

 but unaccompanied with increased redness. 



Incisions were to-day made by the side of the labia in the 

 same three cows, and in like manner a little pus from the second- 

 made wounds was inserted. 



^th. — The second-made wounds are less swelled, and have a 

 more healthy appearance. It will be unnecessary to allude to 

 their condition again. 



10th. — The third inoculated wounds are inflamed. The swell- 

 ing and soreness are quite equal with that of the second at the 

 same distance of time from the inoculation. 



12th.— The wounds have a more unhealthy condition than any 

 of the preceding. The lips are gaping, and the purulent dis^ 

 charge is thin and discoloured, 



14^th. — Slight ulceration is going on. 



l^th. — The incisions are more healthy, and time alone is 

 required to effect their healing ; they will therefore not again 

 be reported. 



Still making these three cows the subjects of experiment, we 

 determined in the next place to inoculate them again ^Niih. fluid 

 from the lung and deep incisions ; this was done, and in each case 

 the ivounds comported themselves as before. 



The sero-purulent discharge was taken as early as possible 

 from these " lung-inoculated " places and used on other cows, in 

 a like manner as it had been in the former experiments on the 

 animals themselves : a similar result attended this procedure. 

 Two sheep were also inoculated with the same purulent fluid at 

 the same time, and the wounds in these animals inflamed and 

 suppurated as in the cows. 



We shall now return to the two cows of which no mention 

 has been made since January 20th, when they were for the last 

 time M?2successfully inoculated by superficially puncturing the 

 skin. On February 1st these animals were inoculated by a 

 deep and roughly made puncture, after the plan we had witnessed 

 in Belgium. At the end of a few days their tails were swollen 

 and tender on the application of pressure. The inflammation 

 increased, and by the eighth day of inoculation pus was being^ 

 discharged from the incisions. A small ulcer formed near to 

 the place of inoculation in one case, and delayed the healing 

 process. In the other case no such event occurred. By the 

 16th the wounds were cicatrizing. 



These being what are described as successful inoculations, we 

 were anxious to inoculate these animals again in the same manner, 

 to ascertain their capability of taking a second time. This was. 

 done. In one cow the effects were as marked as before, in the 

 other the inoculation failed. 



