Inoculation for Pleuro-P neumonia in Cattle. 



261 



The animals were next inoculated with purulent fluid taken 

 from an inoculated sore, the incision being made on one 

 side cf the labia, while on the other side a similar incision, 

 charged with an irritating iiiedicinal agent, was also made, that 

 the effects might be contrasted. The wound in which the pus 

 was placed became inflamed the soonest and to the greatest 

 extent, but so slight was the difference between them, that no 

 person ignorant of the operation would have noted it, or any 

 other peculiarity. 



The operation was next modified by using in one wound the 

 medicinal agent and in the other serous exudation. The order of 

 the phenomena was now reversed, — that is, the wound containing 

 the irritating agent inflamicd the soonest and to the greatest ex- 

 tent ; otherwise no difference was to be detected. 



The final experiment on these animals consisted of inoculating 

 them again with serum and roughly made punctures ; this perfectly 

 succeeded, thus showing that their susceptibility to the local 

 action of the morbific fluid exuded from the lungs was in no way 

 destroyed by the former inoculations. 



Some few experiments have been instituted on other animals, 

 to which we will now briefly allude : — 



Feb. 1th. — Two sheep, a donkey, and a dog were inoculated 

 with serous fluid, and at the same time a heifer which had been 

 three days previously inoculated with sero-purulent matter obtained 

 from an incision on one of the cows. 



^th. — The wounds are inflamed in the clog and the sheep, but 

 not in the donkey or heifer. 



10th. — Inflammation increased in dog and sheep ; incision 

 in heifer slightly inflamed ; no effect in donkey. 



12th. — Incisions suppurating in dog and sheep ; inflammation 

 increased in heifer; a little swelling in the donkey. 



It is sufficient to add, that after this date the inoculated place 

 in the heifer suppurated, the action seemingly in no way being 

 controlled by the previous inoculation. In short, the two wounds 

 comported themselves precisely as erasions of the skin would have 

 done, received on different days by some slight accident. 



The incisions in the dog and sheep healed readily, as did that 

 of the donkey — the latter v/ithout the production of pus. 



Subsequently two other sheep, two pigs, and a second donkey 

 were inoculated in the ordinary manner. In each case inflam- 

 mation, succeeded by suppuration, supervened. The sheep and 

 pigs were inoculated on the inner side of the thigh, while on the 

 opposite thigh in each animal a simple incision of equal dimen- 

 sions, viz. about three-fourths of an inch in length, was made 

 with a clean scalpel for the sake of comparison. These simple 

 wounds healed readily in all the animals, and without suppura- 

 tion, except in one instance where a little pus was formed. 



