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EDWARD A. BURT ON A 



some other factor than repeated folding has aided in their formation. The cavities 

 between the primary and secondary folds of Fig. 17 can now be followed only with 

 great difficulty, and in these cases they seem to be rows of small closed chambers with 

 occasional connecting passages. These systems of cavities are also frequently cut 

 off from reaching quite up to the wall of the arm, which is now developing in 

 the narrow space about the fundament of the arm of the younger stage. All of the 

 chambers in that stage opened into this space. 



The breaking up of the earlier communicating chambers into the many small 

 chambers of this section, seems to indicate that at those places in which changes occurring 

 in the gleba have caused folds to be crowded into close contact, hyphae from the tramal 

 tissue of the one fold, or of each fold, pass into the other and bring about an anastomosis 

 of the folds. Indications of such anastomoses in formation are not infrequent. In his 

 study of Ithyphallus tenuis Fischer 1 pointed out that it may be that anastomosis of 

 neighboring folds is a factor in the formation of the closed chambers of the gleba. 



The deeply staining cells of the hymenial layer are now more elongated and are 

 basidia, bearing a cluster of spores at their outer ends. These basidia already show 

 the series of constrictions which become so singular a feature in later stages. 



Great development of the arm has been taking place. It now fills the whole of 

 the cavity which in Fig. 17 was only partially occupied by its fundament, and is in close 

 contact with the folds of the gleba. Two quite distinct tissues now compose the arm. 

 There is a central mass of fine hyphae running mostly in a longitudinal direction. This 

 tissue is highly gelatinous and, in the double-stained preparations, takes the same 

 orange color that is taken by the gelatinous layer of the peridium, by the tramal tissue, 

 and by the central tissue of the stipe, — all of which are of medullary origin. 



The second tissue of the arm surrounds the gelatinous constituent. It consists 

 of a narrow layer of hyphae connected with the tissue of the cortical plate. These 

 hyphae are branched and irregularly inflated, and are developing into the 

 pseudoparenchyma. This tissue retains in the double-stained preparations the 

 purplish red color given by the carmine, and is sharply distinct from the gelatinous 

 tissue of the arm on the one side and from the gleba on the other. It seems to find 

 conditions for its development most favorable along the surfaces of contact with the 

 gelatinous tissue of the arm and with the gleba rather than midway between these 

 two surfaces. This causes a rather more compact arrangement of this tissue next 

 to these surfaces than in the middle of the space between them. This appearance 

 has been referred to repeatedly by Fischer, and it is probably this which gave him 



1 Fischer : Entwick. der fruchtkorper einiger phalloideen, p. 12, taf. 2, flg. 12. In Annales jardin botanique 



Buitenzorg, vol. 6. 



