498 



EDWARD A. BURT ON A 



From the above observations it may be concluded that near the base of the egg, 

 bundle? of medullary hyphae pass into the fundament of the stipe, branch there perhaps, 

 and become loosely interwoven ; at the place of origin of the arms, these bundles are 

 crowded together more compactly — probably by the extrusions of medullary tissue in 

 forming the gelatinous layers of the peridium — and ascend in six masses, each of 

 which is the fundament of an arm. All of these bundles of hyphae gelatinize and 

 disappear ultimately, and leave empty chambers : hence the course of this tissue from 

 the chambers of the stipe-wall up into the arm is shown by the connection of the 

 cavity of the arm with the cavernous structure of the chambers of the wall of the stipe, 

 reference to which was made in describing the structure of the mature plant. 



The second constituent of the wall of the stipe is, in the stage represented by Figs. 

 22 and 23, more distinctly seen to be in connection with the surrounding sheath of 

 cortical nature than in the younger stage of Figs. 14-17. Its hyphae are differentiating 

 into pseudoparenchyma by the formation of irregular lateral inflations, as described in 

 the case of the wall of the arm. 



Fischer has stated for Clathrus cancellatus and several Phalleae that the hyphae 

 of the chambers radiate outward and form their pseudoparenchyma walls from 

 their swollen tips, contribution to these walls being also made in various Phalleae 

 by similarly swollen tips from medullary hyphae of the main central portion of the 

 stipe on the one side of the stipe-wall and from the primordial tissue (my cortical sheath) 

 on the other. 1 In thick sections there is somewhat of the appearance which he describes 

 and figures repeatedly, but it is due to the packing together of the pseudoparenchyma 

 next to the surface of the gelatinous tissues — to which reference was made in the case 

 of the arm — and to the impossibility of determining with certainty in such sections the 

 real connection between the cell-like pseudoparenchymatous bodies. But even here I 

 can in no case find a hypha from the medullary tissue of the main central cavity of the 

 stipe or from the chambers of its walls making a distinct connection with the 

 pseudoparenchyma — such a connection as is easily seen between the tramal tissue and 

 the basidia. In sections cut 6 2-3 fx thick, it may be seen that the pseudoparenchymatous 

 hyphae run in the plane of the wall and not perpendicularly into it, as would be the case 

 provided they had the origin which Fischer has stated. 



The conditions which I have described stand out still more distinctly, when such 

 a thin section, after removal of its paraffin and after being run down to water but not 

 fixed to the slide, is then treated with a drop of dilute potassium hydrate. The 

 section should be carefully crushed under the cover-glass so as to spread it out somewhat and 



'Fischer: Untersuch. phalloideen, p. 5, 6, and 36, and fig. 26, 27, and 32; and Zur entwicklungsgesch. der 

 fruchtkorper einiger phalloideen, p. 17, and fig. 18. 



