CONTRIBUTIONS FROM THE wini.ky LABORATORY, 



package No. 2, and drop the enclosed wool and powder Into the 

 .solution, giving another stir, ('over the bub with a clean moist 

 cloth, to protect the solution from dust, and keep Ln a warm place 

 ((•.<j. by the side ol the fire), />///, temperatii/re m/ust not exceed 75° to 



HO" /'Vt//r. 



" After twenty four hour;: add tho content;! of packa^o No. M, again 

 .stirring, and allow the mixture to Htand until it, tuniH cloudy. Thil 

 will take place in from twenty four to thirty six hours if the torn 

 perature is suitable. If the Holution has been kept cold further time 



should be. given (not exceeding one or two days) for sufficient growth 



of the bacteria, to produce the cloudiness, as it is useless for inocu 

 lating purposes until it turns cloudy." 



These directions wore rigidly adhered to. 



Tho contents of packet No. I (which contained small quantities of 



cane sugar, potassium phosphate, and magnesium sulphate) were, added 

 to one gallon of recently boiled distilled water, contained in a glazed 

 earthenware vessel, which was scalded out just previous to being usod. 

 When this was quite dissolved the contents of packet No. 2 (the cotton 



wool and earth like particles containing the germs) were, added, and well 

 stirred with a glass rod ; the whole was then covered with a clean towel, 

 to exclude all dust and light, and stood in a propagating pit, where the 



temperature was maintained at 70° Fahr. Twenty four hours, afterwards 



the contents of the third packet (ammonium phosphate) were added, and 

 stirred until the whole was dissolved, the cloth was replaced, and i,he 

 vessel left under the Same conditions, as before. The broth was not very 

 cloudy at the end of forty eight hour;; from the time of starting, but by 

 the end of sixty four hour ; foil March 6) it had become, quite cloudy, 



owing to the development of prodigious cumbers of bacteria, and It was 



then used. 



Inoculation of the Soil. Some soil which had been previou ly dried 



somewhat in the air was taken and well moistened with some ol th« 

 cl udy culture, diluted with its own bulk of water. This was mixed 

 thoroughly with a further bulk of soil, BO that the whole, formed a mass 

 that could be easily spread with a spa.de over the four plot, : which were to 

 la: inoculated (and which had already been dug). This inoculated mil 



after spreading was well raked in. 



Inoculation of the Srr</. The, seed had been previously weighed out 

 into lots of 60 grammes' weight, and forty eight lots were now inoculated 



(March 5). Bach lot was placed separately In a small sieve and dipped 



into the cloudy broth, so that the coat of every seed was thoroughly 



wetted. The process took but a very short time In each case- When fch 



superfluous moisture had been drained away the seeds were spread out in 

 separate lots in the laboratory to dry, the blinds of the room being drawn 

 during the drying process, as light has been found to be injuriou to thl 

 bacteria. 



The weather was not suitable for the .owing of l.he seed lot I ttH df£ 

 after the inoculation, and the seeds were, stored meanwhile in opaque 

 paper bags, numbered to correspond with the row; in the experimental 

 plots. 



VOL* xxxiv. 11 



