KNTOMOPHTIIOREAE OF THE UNITED STATES. 



163 



Conidia broadly ovoid, usually with a single Large oil globule; 22 x '2~> ; , -2o x 10„ ; 

 average 23 x 32/i. Conidiophores, simple. Secondary conidia, like the primary. Resting 

 spores, azygospores, produced Prom spherical hyphal bodies and borne on a neck-like 

 process of variable length. Host- floating on water, or among moss in water. 



Hosts. Diptera: larvae and imagines of Tipulae. 



Habitat Mt. Washington, N. H.; Cullowhee, N. C; Europe (on Culex). 



It is with great hesitation that I have referred to this species a form found in the small 

 brooks which arise in the locality known as the Alpine Garden on Mt. Washington and 

 also very rarely at Cullowhee. In the former locality, the Tipula larvae were very fre- 

 quently met with among moss over which water was running. These larvae were dead 

 having a milky color, and after being placed upon slightly damp moss which absorbed 

 the excess of moisture from them, produced conidia with great rapidity; but in not very 

 considerable quantities. On examination, the body was found in every case to be filled 

 with resting spores in various stages of development, and the conidiophores were present 

 in such small numbers that a " stroma, " such as is described by Sorokin in this species, 

 would not have been apparent. The conidia closely resemble those of E. Grylli, and 

 usually contain a single large oil globule in the centre. The difference in habitat be- 

 tween E. Grylli and the present form is certainly very great, and it is doubtful if E. 

 Grylli would survive soaking in running water for a week or more as is necessarily the 

 case with the Tipula larvae above mentioned. The regular production of resting spores 

 from nearly spherical hyphal bodies is not such as is found in E. Grylli and this is the 

 only morphological difference that I have been able to find between the two. Whether 

 material in good condition and free from resting spores would show the " stroma " of So- 

 rokin, remains to be determined; but judging from the spores figured by this author, 1 as 

 well as by the host and habitat, the present form at least approaches very nearly to E. 

 conglomerata. Sorokin gives no measurements of the conidia that I have been able to 

 find, but should the measurements correspond, the conidia of* the two forms would be in- 

 distinguishable. Sorokin states that the conidium is discharged together with a body of 

 protoplasm from the basidium, a circumstance which, in the examination of dried material, 

 I have not been able to verify in the present instance. 



In the plates of Nowakowski 1 certain of the figures of E. Grylli are drawn from ma- 

 terial on Culex, and this author is inclined to think that E. Grylli and E. conglomerata 

 are the same. The figures referred to do not wholly corroborate this view and it may be 

 mentioned that one among them 2 exhibits the same regular formation of resting spores 

 from spherical hyphal bodies that I have represented in figs. 60 and 61. 



Empusa apiculata, now sp. 



PI. 15, figs. 63-70, 74-75. 



Conidia nearly spherical, with a prominent papillate base, terminating in a short, 

 sharp and abrupt point; 28-30/t X 30-37.«, average 30 X 35 /Jt . Conidiophores simple, some- 

 times with a tendency to become digitate, originating directly or indirectly from nearly 



> l. c. B. ' l- C. B, Plate XI, fig. 9i. 



