220 



GILBERT MORGAN SMITH 



The latter two solutions were made in the same manner as the 

 chrom-acetic-cupric clilorid mixtures, except that a 10 % copper 

 sulphate solution was substituted for the 10 % cupric chlorid 

 solution. 



The value of mercuric chlorid as a preservative was obtained 

 by the use of the mercuric chlorid mixture given by Chamberlain^ 

 to which different copper saks were added. 



(.5) MC Mercuric-chlorid-cupric-chlorid. 



Mercuric chlorid mixture 100 cc. 



10 % cupric chlorid solution 10 cc. 



(G) MC/2 Mercuric-chlorid-cupric-chlorid (half strength). 



Mercuric chlorid mixture 50 cc. 



Water 50 cc. 



10 % cupric chlorid solution 10 cc. 



(7) MS Mereuric-chlorid-copper-sulphate. 



Mercuric chlorid mixture 100 cc. 



10 % copper sulphate solution 10 cc. 



(8) MS/2 Mercuric-chlorid-copper-sulphate (half strength). 



Mercuric chlorid mixture 50 cc. 



Water 50 cc. 



10 % copper sulphate solution 10 cc. 



The ordinary commercial 40 % formalin, reduced to 4 %, formed 

 the basis of another series of mixtures. The effect of different 

 salts of copper, either singly or in combination, was tried with the 

 formalin. The following mixtures were used: 



(9) FAC Formalin, copper acetate and cupric chlorid. 



4 % Formalin 96 cc. 



10 % Cupric chlorid solution 2 cc. 



10 % Copper acetate solution 2 cc. 



(10) 2FAC Double strength formalin, copper acetate and cupric chlorid. 



4 % Formalin 92 cc. 



10 % Cupric chlorid solution 4 cc. 



10 % Copper acetate solution 4 cc. 



(11) 3FAC Triple strength formalin, copper acetate and cupric chlorid. 



4 % Formalin 88 cc. 



10 % Cupric chlorid solution 6 cc. 



10 % Copper acetate solution 6 cc. 



(12) FS Formalin, copper sulphate. 



4 % Formalin 96 cc. 



10 % Copper sulphate 4 cc. 



* Chamberlain, C. J. Loc. cit., p. 248. 



