THK i'KESERVATION OK C'HLOUOI'HYCEAE 221 



(13) '2FS Double strengtli formiilin, coppor siilphiito. 



4% Formalin 92 cc. 



10 % Copper sulphate S cc. 



(14) 3FS Triple strength formalin, copper sulphate. 



4 % Formalin 88 cc. 



10 % Copper sulphate 12 cc. 



(15) FN Formalin, copper nitrate. 



4 % Formalin 96 cc. 



10 % Copper nitrate 4 cc. 



(16) 2FN Double strength formalin, copper nitrate. 



4 % Formalin 92 cc. 



10 % Copper nitrate 8 cc. 



(17) .3FX Tri])le strength formalin, copper nitrate. 



i% Formalin 88 cc. 



10 % Copper nitrate 12 cc. 



The following mixtures h&ve also been recommended for the 

 preservation of green algae: 



(18) M ^Morehouse's mixture' 



Copper acetate 1 gram 



Camphor water 130 cc. 



Glacial acetic acid 20 drops 



Glycerin 260 grams 



In using formula (18) the first three ingredients should be mixed 

 together, the glycerin added, and the whole mass filtered. The 

 Morehouse mixture was also tried in various dilutions, as given 

 below : 



(19) M/2 Morehouse's mixture (half strength). 



Morehouse's mixture 50 cc. 



Water 50 cc. 



(20) M/3 Morehouse's mixture (third strength). 



» Morehouse's mixture 33 cc. 



Water 66 cc. 



(21) M/4 Morehouse's mixture (quarter strength). 



Morehouse's mixture 25 cc. 



Water 75 cc. 



(22) M/5 Morehouse's mixture (fifth strength). 



Morehouse's mixture 20 cc. 



Water 80 cc. 



'Morehouse. Amer. Monthly Mic. Jour. 4 : 234, 1883. Cited by Strasburger, 

 E., Das Botanische Practicum. 3 Aufl., p. 674. 



