222 



GILBERT MORGAN SMITH 



The mixtures of Ripart and Petit/ and that of West, the for- 

 mula for which has been given by Nieuwland,^ have been used 

 extensively for the preservation of green algae. The following is' 

 their composition: 



(23) R and P Ripart and Petit's mixture. 



Camphor water (not saturated) 75 cc. 



Water 7.5 cc. 



Acetic acid (crystals) 1 gram 



Copper acetate 0.3 gram 



Copper nitrate 0.3 gram 



(24) R and P/2 Ripart and Petit's mixture (half strength). 



Ripart and Petit's mixture 50 cc. 



Water 50 cc. 



(25) NW Nieuwland-West mixture. 



Potassium acetate 2% solution 



Copper acetate to color solution 



Amann" has given a complicated formula which has recei^•ed 

 the endorsement of Lagerheim' and'Strasburger.^ The color is 

 retained by the use of copper salts, while the preser\'ative effect 

 is secured by a lacto-phenol solution. Two solutions are described : 

 a weak solution and one of ten-fold strength. In my work I have 

 used the latter diluted to the desired strength. 



The lacto-phenol mixture is made in the following manner: 



Phenol (crystals) 20 grams 



I>actic acid (sp. gr. 1.21) 20 grams 



Glycerin (sp. gr. 1.25) 40 grams 



W'ater 20 grams 



(26) CLP Amann's copper-lacto-phenol. 



Cupric-chlorid (crystals) 2 grams 



Copper acetate (crystals) 2 grams 



Lacto-phenol 96 grams 



(27) 2CLP Double strength copper-lacto-phenol. i 



Cuprie chlorid (crystals) 4 grams 



Copper acetate (crystals) 4 grams 



Lacto-phenol 92 grams 



* See Carnoy, La Biologie Cclhilaire, p. 94. Cited by Lee, A. B., The micro- 

 tomist's vade mecum, 5 ed., p. G5. 



« Nieuwland, J. A. The mounting of algae. Hot. Gaz. 47: 237-238. 1909. 



^ Amann, J. Conservirungsfliissigkeiten und Einschlus.smedien fiir Moose. Chlor- 

 und Cyanophyceen. Zeitschr. f. wiss. Mikrosk. 13: 18-21. 1896. 



' Lagerheim, G. Technische Mittheilungen. Zeitschr. f. wiss. Mikrosk. 14: 

 350-354. 1897. 



* Strasburger, E. Das Botanische Practicum. 3 -\ufl., p. 692. 



