3 



excrement, and an equal amount of fresh water added ; at noon 

 the cans were all aerated by means of a large bicycle-pump, 

 a sponge being tied over the end of the hose to separate the 

 air into fine particles ; at 4 p. m. two gallons of water were 

 siphoned off from the bottom and two gallons of fresh water 

 were put in ; just before retiring the cans were again aerated 

 by means of the air-pump. 



At each place en route where the water was changed it was 

 first tested by placing two fish in a bucket containing the 

 new water at the proper temperature. At El Paso, Texas, 

 only, did the water kill the fish thus treated. After ten 

 minutes the two fish were dead, probably due to the alkali 

 it contained. The water at Los Angeles was good as also the 

 San Francisco water, which was used from the latter place 

 to Honolulu, an abundant supply being carried on the steamer. 

 The water used from El Paso to Los Angeles was taken from 

 the supply tank, filled at San Antonio, Texas. 



Twelve fish died between Galveston and San Francisco and 

 only fifteen between San Francisco and Honolulu. The fish 

 were landed in Honolulu from the S. S. "Alameda" on Sep- 

 tember 15, 1905, the trip from Texas occupying 12 days and 

 27 of the approximate 450 fish were lost. 



The fish were in fine condition on arrival and as prear- 

 ranged by yourself were placed in the breeding ponds pre- 

 pared for them- The temperature of the water about Hono- 

 lulu is almost identical with that where the fish were col- 

 lected, and the appearance of the fish at this writing indicates 

 that they should thrive on the Islands. The fish should be con- 

 fined in the present breeding ponds, where they can be pre- 

 vented from going out to sea or falling prey to other fish until 

 their increased numbers permit general distribution to other 

 localities in the group. 



Very trulv vours, 



J ALVIN SEALE, 

 Assistant, L T . S. Fish Commission. 



Upon the arrival of the steamer the fish were taken at once 

 to Moanalua near Honolulu where, throueh the courtesy of 

 Hon. S. M. Damon, a series of four ponds had been prepared 

 for their reception and breeding. An irrigation ditch led clear 

 water to the ponds through a gate-way sruarded bv wire cloth 

 of a fine mesh. The outlet was guarded in a similar manner. 

 The water was allowed to circulate through three of the ponds 

 but no outlet w r as provided for the fourth in order that the 

 water might remain standing. Two of the ponds were about 



