21 



normal movements on the part of the insect. In order to get 

 some evidence on this point plate cultures were made from the 

 glass tubes in which insects Nos. 63, 64, and 65 were confined 

 during transit from the field. These three insects were Lepto- 

 stylus macula, taken at West Chester in the afternoon of June 

 19. After they had been in the glass tubes for about two hours, 

 they were taken out and transferred to flasks of water. On 

 the following day 1 or 2 cc. of sterile water was poured into 

 each tube, which was shaken vigorously, and the water then 

 emptied into a Petri dish and agar poured over it. It will be 

 noticed from the results obtained (Table III) that a fair 

 percentage of the spores originally adhering to the beetles had 

 been shaken from their bodies by their movements within the 

 glass tubes. 



The time of development of the colonies of the blight fungus 

 in the cultures indicated that almost all of them originated 

 from pycnospores (17), but a very few of them plainly had 

 their origin from ascospores. The colonies obtained in the 

 plates from insects jSFos. 1, 2, 3, and 22, however, started from 

 ascospores, but this is to be expected from the fact that these 

 insects had had access to ascosporic material only. 



TABLE II. 



SUMMARY OF RESULTS OBTAINED FROM CULTURES. 



Kinds of Insects. 



0)^ 



1.2 



* g fcl 



7 



3 



249 



8 



8 



33S.960 



11 



4 



6.715 



2 



0 



6 



1 



1,051 



18 



3 



300 



21 



5 



4.O0O 



2 



0 





75 



24 





21 



5 





54 



19 





Hemiptera 



Coleoptera : 



Leptostylus macula, 



All other beetles : 



Lepidoptera 



Diptera, 



Hymenoptera : 



Ants from the field, 



Ants from laboratory and insectary, 



Spiders 



Grand total 



Minus ants from laboratory and insectary, .. 



Total insects and spiders from the field. 



