Lehman: Penicillium spiculisporum 



271 



(Fig. 30-34). Ascospores are ovate to elliptical, 2.5-4 /x by 

 1 .8-2.8 /x, with walls bearing minute spines (Fig. 35). The 

 spines are visible only under oil immersion, and then to best 

 advantage when the spores have been treated three to five 

 minutes with alcohol carbol fuchsin as if staining bacteria. Only 

 about one per cent, of the ascospores have germinated on any 

 of the various media used. Fig. 37 represents a colony grown 

 from a single ascospore on beef peptone agar kept at 28 0 C. for 

 48 hours. At 72 hours, this colony had attained a diameter of 

 675 /x and had developed a few conidiophores with chains of six 

 to ten spores. 



Penicillium spiculisporum sp. nov. 



Coloniis in agaro Solani tuberosi vel Phaseoli cultis, albis, 

 rare instabiliter sulphureis, floccosis, extendentibus incerte ; re- 

 verso albo aut cremeo. Conidiophoris sparis, ex hyphis aereis 

 orientibus, 10-50 /x X 2-2.5 usitate unum verticillum 1-6 

 basidiorum vel rare 1-2 metulorum vel basidiorum et metulorum 

 gerentibus ; • basidiis 11-16 fi X 1.8-2.5 /x, contractis ad apices; 

 conidiis ovatis, ellipticis vel globosis, hyalinis vel pallide glaucis 

 in massa, 2.5-4 /xX. 1.8-2.5//,, levibus. Peritheciis abundantibus, 

 globosis, primo albis, diende cremeis vel sulphureis, citrinis, flavis 

 vel luteis, 0.4-2 mm., nondehiscentibus, peridiis non parenchy- 

 maticis, ex tribus ordinibus hyphorum, compositis ; ascis globosis, 

 ellipticis vel pyriformis, 7.2-10.8 /x X 6.3-7.7 /x, hyalinis, 6—8 

 sporis ; ascosporis ovatis, ellipticis, hyalinis, spiriulosis, 2.5-4 fx 

 X 1 .8-2.8 ix. Coloniis gelatinam nonliquif acientibus. Odore nullo. 

 Aeris temperatione optima 33-35 0 C. Culturae ex Gossypio her- 

 bacearo Anson Co., N. C, U. S. A. 



Cultural characters 



Potato agar, good growth, spreading colonies with low, white, 

 floccose surface ; glucose or saccharose added, vigorous growth, 

 many perithecia formed ; surface white or transiently yellow, 

 reverse white to cream. 



Potato plugs, vigorous growth soon forming a dense mat of 

 white mycelium, and, later, numerous white, cream and yellow 

 perithecia. 



Bean agar, same as for potato agar but somewhat less vigorous. 



