Notes and Brief Articles 



293 



collections and field observations on Actaea that suggested them. 



The aecia on the infected plants, especially on the stems, pro- 

 duced abundant ascospores for some time. A number of grasses 

 grown from seed in the greenhouse were inoculated with aecio- 

 spores from these cultures and also from aecia collected in the 

 field on Actaea rubra at Ste. Anne de Bellevue. These inocula- 

 tions and the observations in connection with them were made 

 by Miss Margaret Newton, a graduate student of Macdonald 

 College. The results are given in tabular form below. 



Grass Inoculated 



Fjymus canadensis 



L 



Elymus virginicus L 

 Hordeum jubatum L 

 Hystrix patula 



Moench 



Agropyron repens 



(L.) Beauv 



Agropyron Richard- 



sonii Schrad 



Bromus ciliatus L. . . 

 Elymus virginicus L 

 Agropyron Richard- 



sonii 



Hystrix patula 



Elymus canadensis 

 virginicus. 



Source of Material 



Aecispores from 

 culture on Actaea 



Aeciospores from 

 field collection on 

 Actaea 



-Date of 

 Inocu- 

 lation 



Date of 

 Uredinia 



June 13 



Ju 



y 12 



June 22 

 23 



July 21 

 Telia Aug. 13 



No record 

 July 20 



Telia Aug. 13 

 July 19 ! 



Nature of Infection 



Very heavy infection 



Slight infection 



Very heavy infection 



No infection 



Very heavy infection 



No infection 



Very heavy infection 



No infection 



Very heavy infection 



The material from the cultures was studied by Dr. E. B. Mains 

 with the aid of Dr. Arthur's herbarium. He found that the 

 aecial and telial material corresponded to the herbarium materia! 

 of the European Puccinia Actaeae-Elymi Mayor and P. Actaeae- 

 Agropyri^Ed.¥hoh. The urediniospores were somewhat smaller 

 than is usual in Puccinia Clematidis. It seemed as if all these 

 should be considered as belonging to one species, but probably 

 representing several races for whose delimitation further culture 

 work is necessary. They all belong in the species Puccinia 

 Clematidis (DC.) Lagerh. as it has been considered by Arthur in 

 North America. 



These experiments and studies seem to establish the connec- 



