Shear and Dodge : Patellina, Leptothyrium, Peziza 143 



small branches bore an abundance of discocarps of Pezizella 

 oenotherae and also typical pycnidia and sporodochia of the same. 

 During the latter part of August pycnidia began to appear on the 

 larger stems and in September these pycnidia could be found even 

 at the base of the vines. It is not unlikely that the fungus winters 

 over in this condition as many unruptured pycnidia can be found 

 on vines collected in the field in April, too early for them to have 

 developed during the spring. Spores taken form these over- 

 wintered pycnidia germinated readily. . 



It is rather difficult to obtain large quantities of ascospores of 

 Pezizella oenotherae. Noting that spores still within the ascus 

 germinated readily, apothecia were crushed in water and the 

 young asci separated so that when small drops were placed on the 

 surface of agar media the spread of the water was sufficient to 

 separate the asci, care being taken to secure the proper dilution. 

 By marking a number of spots on the petri dishes the separate 

 asci could be located after germination had begun. The asco- 

 spores are so nearly the size of the conidia that they might other- 

 wise be easily confused. In order to avoid this, only asci with 

 germinating spores clearly distinguishable were transferred. Sev- 

 eral dozen single ascus cultures were made at this time and in 

 all about two hundred pure cultures in plates and tubes were 

 obtained from asci. Without exception all produced sporodochia 

 agreeing with Hainesia. Twenty-five single ascus cultures were 

 made from two apothecia on raspberry leaves from Arlington 

 Farm, Virginia, July 23, and no tube cultures from ascospores 

 on the dewberry vines first sprayed with conidia at Cameron, 

 N. C, May 26, 1919, and kept on the ground in the woods at 

 Radnor, Virginia, from April 15 to July 26, 1920. The culture 

 work here summarized proves conclusively the genetic connection 

 between the three forms of fruit bodies described. 



MORPHOLOGY 



The morphological features of the Hainesia stage of this fun- 

 gus have been fairly well described by 'the authors of the various 

 specific names which have been applied to it. Stevens and Peter- 

 son (1916) have noted the variation in form, color and size of 



