56 



Mycologia 



upper stromatic layer with the second one (2) which occupies 

 most of the region normally filled by the spongy parenchyma. 

 The latter is entirely destroyed or one may find a few discon- 

 nected cells here and there throughout the loose white stromatic 

 structure which is 150 to 300 /x thick. This grades rather abruptly 

 into (3) the lower stromatic mass which is dense and black and 

 occupies the under side of the leaf to the utter destruction of the 

 tissues, even the epidermis being destroyed except bits of the 

 cuticle here and there, or near the edge of the spot. This layer 

 is 210 to 230 a thick. 



The perithecia are few or many, depending upon the size of the 

 stroma. They He in the lower stroma, projecting upward into 

 the loose, white middle stroma. In this latter the hyphae are 

 darker and more densely crowded about the perithecial cavity. 

 The perithecia are 400 to 450 high and 300 to 500 /x in diameter. 

 The ostioles are only very slightly papillate. The basal portions 

 of the perithecia appear on cross section of the leaf to be free, 

 but in reality they are bound together by the loose mass of the 

 colorless hyphae making up the middle stromatic layer. Their 

 apical portions are connected by the lower stromatic layer almost 

 as a sort of clypeus. There is no distinct perithecial wall. The 

 very numerous asci arise in the basal portion of the perithecium 

 ^nd are elongate-fusiform, 105-175 X 12-16 /x, tapering gradually 

 to the base and somewhat more abruptly to the rounded apex. 

 • Between the asci are found occasional slender, filiform, septate 

 ( ?) paraphyses which equal the asci in length. These are very 

 numerous around the edge of the mass of asci and line the walls 

 of the upper portion of the perithecial cavity. The eight asco- 

 spores are filiform, 77-87 X 4.7-6.2 fx, dilutely brown, very gran- 

 ular, the color residing in the granules, with a clear spot 

 (vacuole ?) about 5/x in length at the middle of the spore. They 

 are not septate. Toward either end of the ascus they lie singly 

 or doubly, but are in double or triple rows towards the middle. 

 They taper more toward the lower end, being very slightly clavate. 

 They are straight, or curved slightly at the smaller end. 



In the younger spots before the perithecia appear, but persisting 

 even until the maturity of the latter, there appear in the upper 



