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Mycologia 



the same material was sown on Mahonia Aquifolium. In each 

 instance the ready germination of the teliospores was established 

 by a drop culture within twenty-four hours preceding the time of 

 sowing, as is done for all the culture work reported in this and 

 preceding years. In no case was an infection secured. 



The reasons for believing that this rust should be referred to 

 Puccinia poculiformis, in spite of the failure to produce aecia on 

 Berberis or Mahonia, have been briefly stated by Mr. Frank D. 

 Kern, 9 who has also recorded the history of the appearance of 

 the rust in North America, and it is only necessary that the writer 

 affirm his agreement with Mr. Kern's conclusions. 



11. Puccinia Asteris Duby, on Aster arenarioides D. C. 

 Eaton, sent from Salt Lake City, Utah, by Mr. A. O. Garrett, was 

 sown on Aster Drummondii, A. paniculatus, A. multiflorus and 

 Callistephus hortensis, with no infection. This adds little to the 

 solution of the problem whether the leptopuccinial rust on various 

 species of Aster in the different sections of the country are all 

 referable to one species or not. 



12. Uromyces graminicola Burr., on Panicum virgatum L., 

 collected at Stockton, Kans., by Mr. E. Bartholomew, was sown 

 on Althaea rosea, Apios tuberosa, Cacalia reniformis, Callirrhoe 

 involucrata, Decodon verticillatus, Hibiscus ndlitaris, Napaea 

 dioica and Viola cucullata, with no infection. 



13. Uromyces Andropogonis Tracy, on Andropogon glomer- 

 atus (Walt.) B.S.P., collected at Lewes, Del., by Mr. H. S. Jack- 

 son, was sown on Viola cucullata, while a similar collection on 

 the same species of host, sent from Auburn, Ala., by Mr. R. E. 

 Stone, was sown twice on Viola cucullata, and also on Comandra 

 umbellata, Dasystoma flava and Pentstemon hirsutus, all with no 

 infection. From the field observations reported by Dr. John L. 

 Sheldon it seems almost certain that the aecia of this rust occur 

 upon Viola, and the repeated failure of the cultures may have been 

 due to the maturity of the Viola leaves on which the sowings 

 were made. 



Successful cultures supplementing previous work: — 

 The following species of rusts were successfully grown, and the 

 facts supplement what have been obtained from previous cultures 



9 Kern. The Rust of Timothy. Torreya 9: 3-5. 1909. 



