Arthur: Cultures of Uredineae in 1908 245 



cinnamon-brown, paler below, medium thin, 1.5-2.5/x, much thicker 

 at apex, 9-16/x; pedicel nearly colorless, one half to once length of 

 spore. 



On Carex comosa Boott, Lewes, Del., Nov. 15, 1907, H. S. 

 Jackson 1858. 



3. Puccinia on Carex pratensis Drej. An observation by Mr. 

 E. Bethel at Tolland, Colo., July 25, 1907, when aecia on Agoseris 

 were found in very close proximity to rusted Carex pratensis, 

 led to a collection of telial material from the same spot in Octo- 

 ber following, and a successful culture. The teliospores were 

 sown May 13, on a plant of Agoseris glauca, raised from seed pre- 

 viously sent by Mr. Bethel, and pycnia began to show May 19 

 in the greatest abundance. The infection was so heavy that the 

 plant was killed before aecia were formed. 



A study of all available data regarding this species has led to 

 the conclusion that a very large part of all collections of aecia 

 on the various species of Agoseris belong to this species, and not 

 as sometimes assumed to Puccinia Troximontis Peck, which is 

 common on the same hosts, but probably has no aecial stage. A 

 description of the species is appended, but no attempt will be 

 made at this time to cite the numerous known localities or to map 

 out the range, which doubtless embraces much of the western 

 mountainous region. 



Puccinia patruelis sp. nov. 



0. Pycnia amphigenous, rather few, in groups 0.5-1 mm. 

 across, punctiform, inconspicuous, honey-yellow becoming brown- 

 ish, subepidermal, in vertical section globoid, 96-1 25/x, in diameter 

 by 80-100//, high ; ostiolar filaments 30-55//, long. 



1. Aecia amphigenous, numerous in crowded groups 1-4 mm. 

 across, often surrounding groups of pycnia; peridium colorless, 

 rather short, margin finely lacerate; peridial cells rhomboidal, 

 1 8-25/x long, outer wall rather thick, 3-4//,, striate, smooth, inner 

 quite thin, finely verrucose; aeciospores mostly globoid, 13-16 by 

 16-21//,; wall colorless, thin, i/x, very finely verrucose, appearing 

 smooth when wet. 



On Agoseris glauca (Pursh) Greene (Troximon glaucum 

 Pursh), Tolland, Colo., July 25, 1907, E. Bethel. 



II. Uredinia chiefly hypophyllous, scattered, oblong or oval, 

 0.4-0.6 mm. long, rather tardily naked, cinnamon-brown ; uredi- 

 niospores ellipsoid or obovate-ellipsoid, 13-19 by 19-26//; wall 

 golden-brown, moderately echinulate, pores 2, lateral and opposite, 

 slightly superequatorial. 



